Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 3H8] (IgG1,k) to Human PTBP1
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [3H8]
Protein A purified
IHC - Paraffin (5 µg/ml)
Immunofluorescence (10 µg/ml)
Specificity and Use
PTBP1 antibody was raised against pTBP1 (NP_002810, 45 a.a. ~ 145 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control A-431. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
PBS, pH 7.4. Sourced in Ascites.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
PTBP1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm.