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Anti-PTBP1 Antibody (aa2-14) IHC-plus™ LS-B3235

Note: This antibody replaces LS-C55336

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PTBP1 Antibodies

Anti-PTBP1 Antibody (clone 3H8) IHC-plus™ LS-B4399
Mouse Monoclonal [clone 3H8] (IgG1,k) to Human PTBP1
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PTBP1 Antibody IHC-plus™ LS-B13371
Rabbit Polyclonal (IgG) to Human PTBP1
Human, Mouse, Rat
IHC, IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human PTBP1
Human, Monkey, Mouse, Rat, Bovine, Hamster, Pig
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human PTBP1
Goat
Human, Monkey, Mouse, Rat, Bovine, Hamster, Pig (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:8000) & (1 µg/ml)
  • ELISA (1:8000)

Specificity and Use

PTBP1 antibody was raised against synthetic peptide DGIVPDIAVGTKR-C from the N-terminus of human PTBP1 (NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Hamster, Bovine, Pig (100%); Horse, Zebrafish (85%).
LS-E25599 - Lyophilized - 100 µg - $145.00
aa2-14
Human PTBP1. This antibody is expected to recognise all 4 reported isoforms (represented by NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1).
Immunohistochemistry: LS-B3235 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3235 was determined to be 5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About PTBP1

P26599 NM_031991 NP_114368.1

PTBP1 Antibody, HnRNP I Antibody, HNRNPI Antibody, HNRPI Antibody, PTB Antibody, PPTB Antibody, PTB2 Antibody, PTB4 Antibody, RNA-binding protein Antibody, HNRNP-I Antibody, PTB-1 Antibody, PTB-T Antibody, PTB3 Antibody

PTBP1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm.

Immunohistochemistry

Immunohistochemistry
Anti-PTBP1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3235 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-PTBP1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3235 concentration 5 ug/ml.

Western blot

Western blot
Antibody staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
 

Requested From: United States
Date Requested: 12/9/2016

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