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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PSMD12 / Rpn5 Antibody (aa301‑350) LS‑C116758
Rpn5 antibody LS-C116758 is an unconjugated rabbit polyclonal antibody to Rpn5 (PSMD12) from human, monkey, rabbit and other species. Validated for WB.
Catalog
Size
Price
LS-C116758-50
50 µg
Unavailable

Popular PSMD12 / Rpn5 Products

Antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot
Immunohistochemistry of paraffin-embedded human kidney tissue.
Species: Human, Mouse, Rat
Applications: IHC, Western blot
Immunohistochemical analysis of PSMD12 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, Western blot
Western blot of PSMD12 antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
Immunohistochemistry of paraffin-embedded human gastric cancer at dilution 1:100
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

Rpn5 antibody LS-C116758 is an unconjugated rabbit polyclonal antibody to Rpn5 (PSMD12) from human, monkey, rabbit and other species. Validated for WB.
About PSMD12 / Rpn5
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. O00232 NM_002816 NP_002807.1

PSMD12 Antibody, Rpn5 Antibody, p55 Antibody


Specifications

Target
Human PSMD12 / Rpn5
Host
Rabbit
Reactivity
Human, Monkey, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Pig, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
PSMD12 / Rpn5 antibody was raised against synthetic peptide located between aa301-350 of human PSMD12 (Q5RBI3, NP_002807). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Rabbit (100%); Galago, Mouse, Rat, Panda, Dog, Bovine, Bat, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (92%); Hamster, Horse, Xenopus (85%); Zebrafish (84%).
Blocking Peptide
LS-E21144 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116758. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa301-350
Specificity
Human PSMD12 / Rpn5
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

PSMD12 / Rpn5 antibody LS-C116758 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSMD12 / Rpn5 antibody LS-C116758 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PSMD12 / Rpn5 antibody LS-C116758 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSMD12 / Rpn5 antibody LS-C116758 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PSMD12 / Rpn5 antibody LS-C116758 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSMD12 / Rpn5 antibody LS-C116758 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PSMD12 / Rpn5 antibody LS-C116758 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSMD12 / Rpn5 antibody LS-C116758 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/18/2018

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