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PSMC2 / RPT1

proteasome (prosome, macropain) 26S subunit, ATPase, 2

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with several of the basal transcription factors so, in addition to participation in proteasome functions, this subunit may participate in the regulation of transcription. This subunit may also compete with PSMC3 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Gene Name: proteasome (prosome, macropain) 26S subunit, ATPase, 2
Family/Subfamily: ATPase , not assigned-ATPase
Synonyms: PSMC2, Protein MSS1, MSS1, Nbla10058, Protease 26S subunit 7
Target Sequences: NM_002803 NP_002794.1 P35998

Publications (2)

1
Uncommon functional properties of the first piscine 26S proteasome from the Antarctic notothenioid Trematomus bernacchii. Gogliettino M, Balestrieri M, Riccio A, Facchiano A, Fusco C, Palazzo VC, Rossi M, Cocca E, Palmieri G. Bioscience reports. 2016 36: (WB; Human) [Full Text Article] [PubMed:26933238] [PMC:PMC4832319]
2
Unusual Antioxidant Properties of 26S Proteasome Isolated from Cold-Adapted Organisms. Gogliettino M, Cocca E, Fusco C, Agrillo B, Riccio A, Balestrieri M, Facchiano A, Pepe A, Palmieri G. International journal of molecular sciences. 2017 18: (WB; Human) [Full Text Article] [PubMed:28757562] [PMC:PMC5577997]

☰ Filters
Antibodies (40)
Proteins (4)
Over-Expression Lysate (2)
Primary (40)
Recombinant (2)
PSMC2 / RPT1 (44)
Human (40)
Mouse (20)
Rat (11)
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Chimpanzee (3)
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IHC-P (3)
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ICC (3)
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rabbit (35)
mouse (5)
Product Group
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IgG (19)
IgG1 (3)
IgG1,k (2)
monoclonal mc (5)
polyclonal pc (35)
1851CT193.4.40 (1)
4C10-2C8 (1)
MSS1-104 (1)
MSS1-92 (1)
APC (1)
APC Conjugated (2)
Azide-free (1)
Biotin Conjugated (4)
Cy3 Conjugated (1)
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HRP Conjugated (4)
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Unconjugated (22)
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aa1-433 (8)
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aa150-200 (1)
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293T Cells (1)
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Wheat Germ Extract (1)
Purified (1)
PSMC2 / RPT1 Antibody - Western blot of recombinant PSMC2 / RPT1.
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal (aa1-433) Antibody
Mouse, Human
ICC, IP, WB
Unconjugated
100 µl/$309; 200 µl/$351; 500 µl/$491; 1 ml/$561; 2 ml/$755; 5 ml/$1,225
PSMC2 / RPT1 Antibody - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal (aa150-200) Antibody
Mouse, Human
IHC, IHC-P, IP, WB
Unconjugated
50 µl/$460
PSMC2 / RPT1 Antibody - Immunohistochemical analysis of PSMC2 staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$286; 100 µl/$321; 200 µl/$386
PSMC2 / RPT1 Antibody - A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
WB
Unconjugated
100 µl/$397
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$331
PSMC2 / RPT1 Antibody - Immunohistochemistry of paraffin-embedded mouse heart.
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
PSMC2 / RPT1 Antibody - 1:100 staining rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
PSMC2 / RPT1 Antibody - Immunofluorescence of monoclonal antibody to PSMC2 on HeLa cell. [antibody concentration 10 ug/ml]
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PSMC2 / RPT1 Mouse anti-Human Monoclonal (4C10-2C8) Antibody
Human
ELISA, IF, WB
Unconjugated
100 µg/$423
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, IHC, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
PSMC2 / RPT1 Antibody - Immunohistochemistry of paraffin-embedded Human thyroid cancer using PSMC2 Polyclonal Antibody at dilution of 1:25.
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
PSMC2 / RPT1 Antibody - Immunohistochemistry of paraffin-embedded Human cervical cancer using PSMC2 Polyclonal Antibody at dilution of 1:30.
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
PSMC2 / RPT1 Antibody - Immunofluorescence staining of PSMC2 in HEK293 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PSMC2 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal Antibody
Human
ICC, IF
Unconjugated
100 µl/$412
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, WB
Unconjugated
50 µl/$356; 100 µl/$461
PSMC2 / RPT1 Antibody
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PSMC2 / RPT1 Mouse anti-Human Monoclonal Antibody
Human
ELISA, WB
Unconjugated
100 µl/$445
PSMC2 / RPT1 Antibody - All lanes: Anti-PSMC2 at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: U-2OS whole cell lysate Lane 3: Hela whole cell lysate Lane 4: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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PSMC2 / RPT1 Mouse anti-Human Monoclonal (1851CT193.4.40) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$393
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal (aa1-30) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$734
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal (aa1-30) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
200 µl/$952
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal (aa1-30) (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
200 µl/$952
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal (aa1-30) (APC) Antibody
Human
ELISA, WB
APC Conjugated
200 µl/$952
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal (aa1-30) (Azide-free) (HRP) Antibody
Human
ELISA, WB
HRP Conjugated, Azide-free
200 µl/$952
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal (aa1-30) (PE) Antibody
Human
ELISA, WB
PE Conjugated
200 µl/$952
Select
PSMC2 / RPT1 Rabbit anti-Human Polyclonal Antibody
Human
IP, WB
Unconjugated
100 µl/$1,328
PSMC2 / RPT1 Antibody - Immunochemical staining of human PSMC2 in human brain with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.
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PSMC2 / RPT1 Rabbit anti-Human Polyclonal Antibody
Human
IHC-P, WB
Unconjugated
100 µl/$412
PSMC2 / RPT1 Antibody - Western blot: Composite luminograph of (a) HeLa S3 cytosolic preparation, (b) purified 26S proteasome, and (c) human placental proteasome fraction after SDS PAGE followed by blotting onto PVDF membrane and probing with antibody BML-PW8825. Antibody dilution 1:5000 using ECL procedure (1 min exposure).
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PSMC2 / RPT1 Mouse anti-Human Monoclonal (MSS1-104) Antibody
Mouse, Human
WB
Unconjugated
100 µl/$571
Select
PSMC2 / RPT1 Mouse anti-Human Monoclonal (MSS1-92) Antibody
Human
IP
Unconjugated
25 µl/$335
Viewing 1-25 of 44 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).