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Anti-PSMB9 Antibody (aa104-113) LS-C139650

Catalog Size Price
LS-C139650-100 100 µg Unavailable

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30 PSMB9 Antibodies

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IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
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Immunohistochemical analysis of PSMB9 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

100% Guaranteed
Goat Polyclonal to Human PSMB9
Human, Dog, Pig
Western blot, ELISA
Unconjugated

Details

Human PSMB9
Goat
Human, Dog, Pig (tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Bat, Bovine, Hamster, Horse, Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1 - 3 µg/ml)
  • ELISA (1:2000)

Specificity and Use

PSMB9 antibody was raised against synthetic peptide C-RNISYKYRED from an internal region of human PSMB9 / LMP2 (NP_002791.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Panda, Dog, Pig (100%); Monkey, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Horse, Rabbit, Opossum (90%); Trout, Sablefish, Stickleback (80%).
LS-E27484 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C139650. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa104-113
Human PSMB9 / LMP2.
Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Approx 22kD band observed in Human Thymus lysates (calculated MW of 23.3kD according to NP_002791.1). Recommended concentration: 1-3 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About PSMB9

P28065 NM_002800 NP_002791.1

PSMB9 Antibody, Beta1i Antibody, Macropain chain 7 Antibody, Proteasome-related gene 2 Antibody, Proteasome beta 9 Antibody, Proteasome subunit beta type-9 Antibody, PSMB6i Antibody, Low molecular mass protein 2 Antibody, Proteasome subunit beta 6i Antibody, Proteasome subunit beta-1i Antibody, LMP2 Antibody, Proteasome chain 7 Antibody, RING12 Antibody

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.

Western blot

PSMB9 antibody (1 ug/ml) staining of Human Thymus lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PSMB9 antibody (1 ug/ml) staining of Human Thymus lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 4/21/2018

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