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Anti-PSMB8 / LMP7 Antibody (aa52-276) LS-C186312

Catalog Size Price
LS-C186312-100 100 µl (0.38 mg/ml) Unavailable

Related Products

31 PSMB8 / LMP7 Antibodies

Most Popular PSMB8 / LMP7 Antibodies

Anti-PSMB8 / LMP7 Antibody (clone 1B3) LS-C133416
Mouse Monoclonal [clone 1B3] (IgG2a,k) to Human PSMB8 / LMP7
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunoperoxidase of monoclonal antibody to PSMB8 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml].
Anti-PSMB8 / LMP7 Antibody (clone 1A5) LS-C169405
Mouse Monoclonal [clone 1A5] (IgG1) to Human PSMB8 / LMP7
Human
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
IHC of paraffin-embedded intima cancer tissues using PSMB8 mouse monoclonal antibody with DAB staining.
Anti-PSMB8 / LMP7 Antibody (aa75-272, FITC) LS-C302416
Rabbit Polyclonal (IgG) to Mouse PSMB8 / LMP7
Mouse
Western blot
FITC Conjugated
Western blot of recombinant LMP7 / PSMB8.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PSMB8 / LMP7 Antibody LS-C482808
Rabbit Polyclonal to Human PSMB8 / LMP7
Human
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of PSMB8 staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-PSMB8 / LMP7 Antibody IHC-plus™ LS-B15635
Rabbit Polyclonal (IgG) to Human PSMB8 / LMP7
Human, Mouse, Rat
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry of paraffin-embedded human colon tissue, at a dilution of 1:100.

100% Guaranteed
Rabbit Polyclonal (IgG) to Human PSMB8 / LMP7
Human
IHC, IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human PSMB8 / LMP7
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Bovine, Dog, Pig (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

PSMB8 / LMP7 antibody was raised against recombinant fragment corresponding to a region within amino acids 52 and 276 of PSMB8 (SwissProt P28062). Percent identity by BLAST analysis: Human (100%); Mouse, Pig, Rat (94%); Bovine (93%); Dog (91%); Xenopus (83%); Zebrafish (82%).
aa52-276
Human LMP7 / PSMB8
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About PSMB8 / LMP7

P28062 NM_004159 NP_004150.1

PSMB8 Antibody, D6S216 Antibody, ALDD Antibody, Low molecular mass protein 7 Antibody, LMP7 Antibody, JMP Antibody, Proteasome subunit beta 5i Antibody, Proteasome subunit beta type-8 Antibody, PSMB5i Antibody, Macropain subunit C13 Antibody, Y2 Antibody, NKJO Antibody, Protease component C13 Antibody, Proteasome component C13 Antibody, Proteasome subunit beta-5i Antibody, Proteasome-related gene 7 Antibody, RING10 Antibody, Beta5i Antibody, D6S216E Antibody, Low molecular weight protein 7 Antibody, Proteasome subunit Y2 Antibody

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.

Immunohistochemistry

IHC of paraffin-embedded Cal27 Xenograft using PSMB8 antibody at 1:100 dilution.
IHC of paraffin-embedded Cal27 Xenograft using PSMB8 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using PSMB8 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using PSMB8 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Raji. 12% SDS PAGE. LMP7 / PSMB8 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Raji. 12% SDS PAGE. LMP7 / PSMB8 antibody diluted at 1:1000.

Requested From: 
Date Requested: 4/26/2018

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