Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Rabbit Polyclonal (IgG) to Human PSMB8 / LMP7
IHC, IHC - Paraffin, ICC, Western blot
Human PSMB8 / LMP7
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Bovine, Dog, Pig (at least 90% immunogen sequence identity)
IHC - Paraffin (1:100 - 1:1000)
ICC (1:100 - 1:1000)
Western blot (1:500 - 1:3000)
Specificity and Use
PSMB8 / LMP7 antibody was raised against recombinant fragment corresponding to a region within amino acids 52 and 276 of PSMB8 (SwissProt P28062). Percent identity by BLAST analysis: Human (100%); Mouse, Pig, Rat (94%); Bovine (93%); Dog (91%); Xenopus (83%); Zebrafish (82%).
Human LMP7 / PSMB8
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.