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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PSMB8 / LMP7 Antibody (aa52‑276) LS‑C186312
LMP7 antibody LS-C186312 is an unconjugated rabbit polyclonal antibody to human LMP7 (PSMB8). Validated for ICC, IHC and WB.
Catalog
Size
Price
LS-C186312-100
100 µl (0.38 mg/ml)
Unavailable

Popular PSMB8 / LMP7 Products

Immunoperoxidase of monoclonal antibody to PSMB8 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml].
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
IHC of paraffin-embedded intima cancer tissues using PSMB8 mouse monoclonal antibody with DAB staining.
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA
Anti-LMP7 / PSMB8 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot of recombinant LMP7 / PSMB8.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Western blot
Immunohistochemistry of paraffin-embedded human colon tissue, at a dilution of 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

LMP7 antibody LS-C186312 is an unconjugated rabbit polyclonal antibody to human LMP7 (PSMB8). Validated for ICC, IHC and WB.
About PSMB8 / LMP7
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. P28062 NM_004159 NP_004150.1

PSMB8 Antibody, D6S216 Antibody, ALDD Antibody, Low molecular mass protein 7 Antibody, LMP7 Antibody, JMP Antibody, Proteasome subunit beta 5i Antibody, Proteasome subunit beta type-8 Antibody, PSMB5i Antibody, Macropain subunit C13 Antibody, Y2 Antibody, NKJO Antibody, Protease component C13 Antibody, Proteasome component C13 Antibody, Proteasome subunit beta-5i Antibody, Proteasome-related gene 7 Antibody, RING10 Antibody, Beta5i Antibody, D6S216E Antibody, Low molecular weight protein 7 Antibody, Proteasome subunit Y2 Antibody


Specifications

Target
Human PSMB8 / LMP7
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine, Dog, Pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PSMB8 / LMP7 antibody was raised against recombinant fragment corresponding to a region within amino acids 52 and 276 of PSMB8 (SwissProt P28062). Percent identity by BLAST analysis: Human (100%); Mouse, Pig, Rat (94%); Bovine (93%); Dog (91%); Xenopus (83%); Zebrafish (82%).
Epitope
aa52-276
Specificity
Human LMP7 / PSMB8
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Cal27 Xenograft using PSMB8 antibody at 1:100 dilution.
IHC of paraffin-embedded Cal27 Xenograft using PSMB8 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using PSMB8 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using PSMB8 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Raji. 12% SDS PAGE. LMP7 / PSMB8 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Raji. 12% SDS PAGE. LMP7 / PSMB8 antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded Cal27 Xenograft using PSMB8 antibody at 1:100 dilution.
IHC of paraffin-embedded Cal27 Xenograft using PSMB8 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using PSMB8 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using PSMB8 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Raji. 12% SDS PAGE. LMP7 / PSMB8 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Raji. 12% SDS PAGE. LMP7 / PSMB8 antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded Cal27 Xenograft using PSMB8 antibody at 1:100 dilution.
IHC of paraffin-embedded Cal27 Xenograft using PSMB8 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using PSMB8 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using PSMB8 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Raji. 12% SDS PAGE. LMP7 / PSMB8 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Raji. 12% SDS PAGE. LMP7 / PSMB8 antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded Cal27 Xenograft using PSMB8 antibody at 1:100 dilution.
IHC of paraffin-embedded Cal27 Xenograft using PSMB8 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using PSMB8 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using PSMB8 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Raji. 12% SDS PAGE. LMP7 / PSMB8 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Raji. 12% SDS PAGE. LMP7 / PSMB8 antibody diluted at 1:1000.


Requested From: 
Date Requested: 6/20/2018

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