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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PSMB8 / LMP7 Antibody (aa263‑276) IHC‑plus™ LS‑B9206
Note: This antibody replaces LS-C112796
LMP7 antibody LS-B9206 is an unconjugated goat polyclonal antibody to human LMP7 (PSMB8). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B9206-50
50 µg (0.5 mg/ml)
Unavailable

Popular PSMB8 / LMP7 Products

Immunoperoxidase of monoclonal antibody to PSMB8 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml].
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Western blot of recombinant LMP7 / PSMB8.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Immunohistochemistry of paraffin-embedded human colon tissue, at a dilution of 1:100.
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Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

LMP7 antibody LS-B9206 is an unconjugated goat polyclonal antibody to human LMP7 (PSMB8). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About PSMB8 / LMP7
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. P28062 NM_004159 NP_004150.1

PSMB8 Antibody, D6S216 Antibody, ALDD Antibody, Low molecular mass protein 7 Antibody, LMP7 Antibody, JMP Antibody, Proteasome subunit beta 5i Antibody, Proteasome subunit beta type-8 Antibody, PSMB5i Antibody, Macropain subunit C13 Antibody, Y2 Antibody, NKJO Antibody, Protease component C13 Antibody, Proteasome component C13 Antibody, Proteasome subunit beta-5i Antibody, Proteasome-related gene 7 Antibody, RING10 Antibody, Beta5i Antibody, D6S216E Antibody, Low molecular weight protein 7 Antibody, Proteasome subunit Y2 Antibody


Specifications

Target
Human PSMB8 / LMP7
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.01 - 0.03 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PSMB8 / LMP7 antibody was raised against synthetic peptide C-DVSDLLHQYREANQ from the C-terminus of human PSMB8 (NP_004150.1; NP_683720.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey (100%); Gibbon, Marmoset, Elephant (93%); Goat, Panda, Bat, Bovine, Horse, Pig (86%).
Blocking Peptide
LS-E27091 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B9206. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa263-276
Specificity
Human PSMB8. This antibody is expected to recognize both reported isoforms (NP_004150.1; NP_683720.2).
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 22kDa band observed in Human Liver, Lung and Lymph Node lysates (calculated MW of 29.8kDa according to precursor NP_004150.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schwarz et al, J Immunol. 2000 Jul 15;165(2):768-78. PMID: 10878350. Recommended concentration: 0.01-0.03 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-LMP7 / PSMB8 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-LMP7 / PSMB8 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

PSMB8 antibody (0.01 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PSMB8 antibody (0.01 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-LMP7 / PSMB8 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-LMP7 / PSMB8 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

PSMB8 antibody (0.01 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PSMB8 antibody (0.01 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-LMP7 / PSMB8 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-LMP7 / PSMB8 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

PSMB8 antibody (0.01 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PSMB8 antibody (0.01 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-LMP7 / PSMB8 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-LMP7 / PSMB8 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

PSMB8 antibody (0.01 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PSMB8 antibody (0.01 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/23/2018

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