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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PSMB3 Antibody (aa71‑120) LS‑C102705 Best Seller
PSMB3 antibody LS-C102705 is an unconjugated rabbit polyclonal antibody to PSMB3 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C102705-50
50 µg (1 mg/ml)
Unavailable

Popular PSMB3 Products

Anti-PSMB3 antibody IHC staining of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9527 concentration 10 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Xenopus, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human, Mouse, Rat, Rabbit
Applications: IHC, Western blot
All lanes: Anti-PSMB3 Antibody (Center) at 1:2000 dilution. Lane 1: K562 whole cell lysates. Lane 2: HeLa whole cell lysates. Lane 3: Jurkat whole cell lysates. Lane 4: 293T/17 whole cell lysates. Lane 5: NIH/3T3 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 23 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Human, Mouse
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemistry of paraffin-embedded human skin tissue at dilution 1:100
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

PSMB3 antibody LS-C102705 is an unconjugated rabbit polyclonal antibody to PSMB3 from human, mouse, rat and other species. Validated for WB.
About PSMB3
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. P49720 NM_002795 NP_002786.2

PSMB3 Antibody, HC10-II Antibody, Macropain theta chain Antibody, Proteasome chain 13 Antibody, Proteasome theta chain Antibody, Proteasome subunit beta type-3 Antibody, C10-ii Antibody, Proteasome component C10-II Antibody


Specifications

Target
Human PSMB3
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Guinea pig, Chicken, Drosophila (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
PSMB3 antibody was raised against synthetic peptide located between aa71-120 of human PSMB3 (P49720, NP_002786). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Mouse, Rat, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Arabidopsis (100%); Guinea pig, Turkey, Chicken, Lizard, Smelt, Stickleback (92%); Drosophila, Rice, Grape (90%); Zebra finch, Xenopus, Trout, Catfish, Salmon, Zebrafish, Sea squirt, Slime mold (85%).
Epitope
aa71-120
Specificity
Human PSMB3
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Reconstitute with 50ul sterile ddH2O.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

PSMB3 antibody LS-C102705 Western blot of Fetal Muscle lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSMB3 antibody LS-C102705 Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PSMB3 antibody LS-C102705 Western blot of Fetal Muscle lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSMB3 antibody LS-C102705 Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PSMB3 antibody LS-C102705 Western blot of Fetal Muscle lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSMB3 antibody LS-C102705 Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PSMB3 antibody LS-C102705 Western blot of Fetal Muscle lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSMB3 antibody LS-C102705 Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/25/2018

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