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Anti-PRSS3 / Trypsin 3 Antibody LS-C132707

Catalog Size Price
LS-C132707-100 100 µg Unavailable

Most Popular PRSS3 / Trypsin 3 Antibodies

Anti-PRSS3 / Trypsin 3 Antibody (aa16-246) LS-C39954
Goat Polyclonal (IgG) to Mouse PRSS3 / Trypsin 3
Mouse
Western blot, ELISA
Unconjugated
Anti-PRSS3 / Trypsin 3 Antibody (aa13-40) LS-C162196
Rabbit Polyclonal to Human PRSS3 / Trypsin 3
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-PRSS3 / Trypsin 3 Antibody (aa143-171) LS-C324335
Rabbit Polyclonal (IgG) to Human PRSS3 / Trypsin 3
Human
IHC, Western blot, Flow Cytometry, ELISA
Unconjugated
Anti-PRSS3 / Trypsin 3 Antibody (aa20-48) LS-C324337
Rabbit Polyclonal (IgG) to Human PRSS3 / Trypsin 3
Human
IHC, Western blot, Flow Cytometry, ELISA
Unconjugated
Anti-PRSS3 / Trypsin 3 Antibody (aa71-120) IHC-plus™ LS-B11527
Rabbit Polyclonal to Human PRSS3 / Trypsin 3
Human, Monkey, Rat, Chicken
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed
Rat Monoclonal (IgG2a) to Human PRSS3 / Trypsin 3
Human
Western blot, Neutralization
Unconjugated

Details

Human PRSS3 / Trypsin 3
Rat
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • Western blot (1 - 2 µg/ml)
  • Neutralization

Specificity and Use

PRSS3 / Trypsin 3 antibody was raised against nS0-derived recombinant human rhTrypsin-3.
Recognizes both pro and mature forms of human trypsin 3. Species sequence homology: chimpanzee: 96%, rat: 76%, mouse: 74%, dog: 72%. and chicken: 67%. Does not cross-react with rhTrypsin-1 or rhTrypsin-2.
Suitable for use in Neutralization and Western Blot. Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by the antibody at approximately 0.15 ug/ml (IC 50) under conditions in which the enzyme was present at 0.03 ug/ml and the substrate concentration was 10uM. Considering the molecular masses of the enzyme (26kD) and the antibody (150kD), IC 50 was achieved at approximately 1:1 molar ratio of the antibody to the enzyme. Western blot:1-2 ug/ml. Using a colorimetric detection system, the detection limit for rhTrypsin-3 is ~25 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Reducing conditions are not recommended.

Packaging

Lyophilized from PBS, 5% trehalose
Sterile buffer.
Lyophilized powder may be stored at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C.
For research use only.

About PRSS3 / Trypsin 3

P35030 NM_002771 NP_002762.2

PRSS3 Antibody, Brain trypsinogen Antibody, Mesotrypsin Antibody, PRSS4 Antibody, Serine protease 4 Antibody, Serine protease 3 Antibody, TRY4 Antibody, Trypsin III Antibody, Trypsin IV Antibody, Trypsinogen 4 Antibody, MTG Antibody, Trypsinogen 5 Antibody, Pancreatic trypsinogen III Antibody, Protease, serine, 3 Antibody, Trypsin-3 Antibody, Mesotrypsinogen Antibody, T9 Antibody, TRY3 Antibody, Trypsinogen IV Antibody

PRSS3 / Trypsin 3 is a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene is localized to the locus of T cell receptor beta variable orphans on chromosome 9. Four transcript variants encoding different isoforms have been described for this gene.

Requested From: 
Date Requested: 6/29/2017

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