Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Human Proline Transporter
Western blot, Immunoprecipitation, ELISA
Human Proline Transporter
Human, Rat (tested or 100% immunogen sequence identity)
Western blot (1:1000 - 1:5000)
Specificity and Use
A 24 AA peptide, near the C-terminal, cytoplasmic domain was synthesized, coupled to KLH.
The 24-aa rat peptide used in this study shows 100% homology with the human transporter. Antibodies made to this epitope have been shown to cross-react with human Proline transporter. Actual cross-reactivity of antibodies in all species is not established.
Suitable for use in ELISA, Western Blot, Immunoprecipitation. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure using ECL. An antibody raised against this epitope has been shown to recognize a ~68kD band in rat and human brain. Deglycosylation reduces the MW to approx. 53kD. ELISA (1:100K using 50-100 ng PROL11 control peptide/well. Immunoprecipitations We recommend the use of affinity pure antibodies at 2-10 ug affinity pure antibody.
PBS, pH 7.4, 0.1% BSA
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.