Human Proliferation Marker IPO-38

Mouse

Human (tested or 100% immunogen sequence identity)

IgM Monoclonal

IPO-38

Unconjugated

Protein A affinity chromatography

Unmodified

A BALB/c mouse was immunized with spleen cells of a human patient with hairy cell leukemia.

Reacts with a 14 -16 kDa protein as demonstrated by western blot analysis on the Raji cell line. This antigen was detected after 12h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes. Cross reactivity is likely to occur with mouse and rat. Reactivity with other sources has not been determined.

• IHC
• IHC - Paraffin (10 µg/ml)
• IHC - Frozen
• Western blot (1:500 - 1:2000)
• ELISA (1:10000 - 1:50000)

This antibody is suitable for ELISA, immunoprecipitation, western blotting and immunohistochemistry (frozen and formal in/paraffin). The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells (paraformaldehyde fixed, Triton X-1 00 permeabilized). An increase of 400% is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemide). IPO-38 does not block the binding of the Ki-67 antibody. Tonsilar tissue is typically used as a positive control.

0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide

Aliquot and store at -20°C or below for extended storage. Avoid freeze / thaw cycles. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.