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Anti-PRMT6 Antibody IHC-plus™ LS-B118

Note: This antibody replaces LS-C777

Ordering

Wt. Vol. Conc. Price
- 50 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PRMT6 Antibodies

Anti-PRMT6 Antibody IHC-plus™ LS-B118
Rabbit Polyclonal (IgG) to Human PRMT6
Human, Monkey, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PRMT6 Antibody LS-C332060
Rabbit Polyclonal (IgG) to Human PRMT6
Human
IHC, Western blot
Unconjugated
Anti-PRMT6 Antibody LS-C409363
Rabbit Polyclonal (IgG) to Human PRMT6
Human, Mouse, Rat
IHC, Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human PRMT6
Human, Monkey, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Human PRMT6
Rabbit
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (1:100)
  • Western blot (1:500 - 1:1000)

Specificity and Use

PRMT6 antibody was raised against this antibody was developed a synthetic peptide corresponding to amino acids 23-43 (C-EEDGAEREAALERPRRTKRER) of human PRMT6, GenBank no. gb|AAH73866.1|. HeLa cell lysate (IMGENEX, cat. no. 40161) can be used as positive control. This antibody also cross reacts with mouse cell line (RAW). Percent identity by BLAST analysis: Human, Monkey, Marmoset (100%); Orangutan (95%); Elephant (86%); Horse (81%).
Synthetic peptide of human PRMT6.
Immunohistochemistry: LS-B118 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B118 was determined to be 1:100.

Packaging

PBS, 0.05% BSA, 0.05% sodium azide
Long term: -70°C; Short term: +4°C
For research use only.

About PRMT6

Q96LA8 NM_018137 AAK85733.1

PRMT6 Antibody, Arginine methyltransferase 6 Antibody, HRMT1L6 Antibody

Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a.

Immunohistochemistry

Immunohistochemistry
Anti-PRMT6 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B118 dilution 1:100.

Western blot

Western blot
Western blot of PRMT6 in cell lysates from HeLa cells using antibody at 1:500 dilution. Twenty microgram of HeLa cell lysate was loaded per well of a mini gel.

Requested From: United States
Date Requested: 12/6/2016

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