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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PRMT6 Antibody (aa42‑53) LS‑C155021
PRMT6 antibody LS-C155021 is an unconjugated goat polyclonal antibody to PRMT6 from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C155021-100
100 µg (0.5 mg/ml)
Unavailable

Popular PRMT6 Products

Anti-PRMT6 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B118 dilution 1:100.
Species: Human, Monkey, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Dog, Guinea pig, Horse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Immunoprecipitation
Detection of Human PRMT6 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PRMT6 used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.
Species: Human, Bovine, Dog, Horse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemistry stain of paraffin-embedded human breast cancer using PRMT6 mouse monoclonal antibody (1:200).
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot
Immunohistochemistry of paraffin-embedded human testis tissue at dilution of 1:100
Species: Human
Applications: IHC, Immunofluorescence, ELISA

Product Description

PRMT6 antibody LS-C155021 is an unconjugated goat polyclonal antibody to PRMT6 from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
About PRMT6
Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. Q96LA8 NM_018137 AAK85733.1

PRMT6 Antibody, Arginine methyltransferase 6 Antibody, HRMT1L6 Antibody


Specifications

Target
Human PRMT6
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Guinea pig (tested or 100% immunogen sequence identity)
Predicted
Bat, Bovine, Dog, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Immunogen
PRMT6 antibody was raised against synthetic peptide ERDQLYYECYSD from an internal region of human PRMT6 (NP_060607.2). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Guinea pig (100%); Bat, Bovine, Dog, Horse (92%); Opossum (83%).
Blocking Peptide
LS-E27912 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C155021. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa42-53
Specificity
Human PRMT6.
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 45kD band observed in lysates of cell line MCF7 (calculated MW of 41.9kD according to NP_060607.2). Recommended concentration: 0.5-2 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

PRMT6 antibody (0.5 ug/ml) staining of MCF7 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PRMT6 antibody (0.5 ug/ml) staining of MCF7 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

PRMT6 antibody (0.5 ug/ml) staining of MCF7 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PRMT6 antibody (0.5 ug/ml) staining of MCF7 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

PRMT6 antibody (0.5 ug/ml) staining of MCF7 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PRMT6 antibody (0.5 ug/ml) staining of MCF7 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

PRMT6 antibody (0.5 ug/ml) staining of MCF7 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PRMT6 antibody (0.5 ug/ml) staining of MCF7 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/18/2018

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