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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PRMT6 Antibody (aa275‑324) LS‑C110491
PRMT6 antibody LS-C110491 is an unconjugated rabbit polyclonal antibody to PRMT6 from human, dog and monkey. Validated for WB.
Catalog
Size
Price
LS-C110491-50
50 µg
Unavailable

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Product Description

PRMT6 antibody LS-C110491 is an unconjugated rabbit polyclonal antibody to PRMT6 from human, dog and monkey. Validated for WB.
About PRMT6
Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. Q96LA8 NM_018137 AAK85733.1

PRMT6 Antibody, Arginine methyltransferase 6 Antibody, HRMT1L6 Antibody


Specifications

Target
Human PRMT6
Host
Rabbit
Reactivity
Human, Monkey, Dog (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Guinea pig, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
PRMT6 antibody was raised against synthetic peptide located between aa275-324 of human PRMT6 (A3KME1, NP_060607). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset (100%); Mouse, Rat, Elephant, Panda, Horse, Guinea pig (92%); Dog, Bovine, Bat, Opossum (85%).
Blocking Peptide
LS-E14625 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110491. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa275-324
Specificity
Human PRMT6
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

PRMT6 antibody LS-C110491 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PRMT6 antibody LS-C110491 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PRMT6 antibody LS-C110491 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PRMT6 antibody LS-C110491 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PRMT6 antibody LS-C110491 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PRMT6 antibody LS-C110491 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PRMT6 antibody LS-C110491 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PRMT6 antibody LS-C110491 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/23/2018

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