PRMT5 antibody LS-C9710 is an unconjugated rabbit polyclonal antibody to PRMT5 from human, mouse, rat and other species. Validated for IP and WB.
Human, Monkey, Mouse, Rat, Bat, Dog, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Bovine, Chicken, Xenopus (at least 90% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot
- (applications tested for the base form of this product only)
PRMT5 antibody was raised against kLH-conjugated, synthetic peptide corresponding to aa60-79 (KNRPGPQTRSDLLLSGRDWN) of human protein arginine methyltransferase 5, SKB1 homolog. The immunizing sequence is identical in mouse. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Horse, Rabbit (100%); Xenopus, Bovine (95%); Chicken, Opossum (90%); Zebrafish, Pufferfish (85%); Medaka (80%).
Recognizes PRMT5, Mr ~70kD. Species cross-reactivity: Human and mouse.
Suitable for use in Western Blot and Immunoprecipitation. Immunoprecipitation: An independent laboratory has reported that this antibody immunoprecipitates active PRMT5 from HeLa cell lysate.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.