Human, Monkey, Mouse, Rat, Bat, Dog, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Bovine, Chicken, Xenopus (at least 90% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot
- (applications tested for the base form of this product only)
PRMT5 antibody was raised against kLH-conjugated, synthetic peptide corresponding to aa60-79 (KNRPGPQTRSDLLLSGRDWN) of human protein arginine methyltransferase 5, SKB1 homolog. The immunizing sequence is identical in mouse. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Horse, Rabbit (100%); Xenopus, Bovine (95%); Chicken, Opossum (90%); Zebrafish, Pufferfish (85%); Medaka (80%).
Recognizes PRMT5, Mr ~70kD. Species cross-reactivity: Human and mouse.
Suitable for use in Western Blot and Immunoprecipitation. Immunoprecipitation: An independent laboratory has reported that this antibody immunoprecipitates active PRMT5 from HeLa cell lysate.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.