Target
Human PRMT3
Synonyms
PRMT3 | Arginine N-methyltransferase 3 | HRMT1L3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence DPKTLISEPCGIKH, from the internal region of the protein sequence according to NP_005779.1NP_001138638.1NP_001138639.1.
Specificity
Human PRMT3. This antibody is expected to recognize all reported isoforms (NP_005779.1; NP_001138638.1; NP_001138639.1).
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 70kD band observed in lysates of cell line MCF7 (calculated MW of 59.9kD according to NP_005779.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Swiercz et al, J Biol Chem. 2007 Jun 8;282(23):16917-23. PMID: 17439947). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee