Human, Mouse, Rat, Bovine, Dog, Hamster, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Monkey, Xenopus (at least 90% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot
- (applications tested for the base form of this product only)
PRMT1 antibody was raised against kLH-conjugated, synthetic peptide corresponding to aa298-318 (KTGEEIFGTIGMRPNAKNNRD) of human protein arginine methyltransferase 1 (accession number CAA71765). The immunizing sequence is identical in mouse. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Mouse, Rat, Hamster, Elephant, Panda, Porcine, Dog, Bovine, Horse, Rabbit, Opossum (100%); Xenopus (95%); Monkey (90%); Zebrafish, Stickleback, Pufferfish (86%); Salmon (81%).
Recognize the PRMT1 doublet, Mr ~42-44kD. A non-specific protein was also detected, Mr ~120kD. Species cross-reactivity: Human and mouse.
Suitable for use in Western Blot. Western Blot Analysis: 0.1-0.5 ug/ml of this lot detected PRMT1 in RIPA lysates from Jurkat and 3T3/A31, NIH 3T3, HeLa, and mouse ES cells.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.