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Antibodies > PRLR / Prolactin Receptor > LS-C313207
 
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Catalog Number Size Price
LS-C313207-10 10 µg $318 
LS-C313207-100 100 µg (0.5 mg/ml) $470 
PRLR / Prolactin Receptor Antibody - PRLR / Prolactin Receptor antibody. IHC(P): Rat Testis Tissue.
PRLR / Prolactin Receptor Antibody - PRLR / Prolactin Receptor antibody. IHC(P): Human Breast Cancer Tissue.
PRLR / Prolactin Receptor Antibody - Western blot analysis of PRLR using anti-PRLR antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRLR antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRLR at approximately 95KD. The expected band size for PRLR is at 70KD.
PRLR / Prolactin Receptor Antibody - Western blot analysis of PRLR using anti-PRLR antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRLR antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRLR at approximately 90KD. The expected band size for PRLR is at 70KD.
PRLR / Prolactin Receptor Antibody - WB of PRLR / Prolactin Receptor antibody. Lane 1: HELA Cell Lysate. Lane 2: MCF-7 Cell Lysate.
PRLR / Prolactin Receptor Antibody - PRLR / Prolactin Receptor antibody. IHC(P): Rat Testis Tissue.
PRLR / Prolactin Receptor Antibody - PRLR / Prolactin Receptor antibody. IHC(P): Human Breast Cancer Tissue.
PRLR / Prolactin Receptor Antibody - Western blot analysis of PRLR using anti-PRLR antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRLR antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRLR at approximately 95KD. The expected band size for PRLR is at 70KD.
PRLR / Prolactin Receptor Antibody - Western blot analysis of PRLR using anti-PRLR antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRLR antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRLR at approximately 90KD. The expected band size for PRLR is at 70KD.
PRLR / Prolactin Receptor Antibody - WB of PRLR / Prolactin Receptor antibody. Lane 1: HELA Cell Lysate. Lane 2: MCF-7 Cell Lysate.
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Polyclonal Rabbit anti‑Mouse PRLR / Prolactin Receptor Antibody (aa591‑605, IHC, WB) LS‑C313207

Polyclonal Rabbit anti‑Mouse PRLR / Prolactin Receptor Antibody (aa591‑605, IHC, WB) LS‑C313207

Submit Review LSBio Guarantee
Note: This antibody replaces LS-C358027
Antibody:
PRLR / Prolactin Receptor Rabbit anti-Mouse Polyclonal (aa591-605) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313207-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PRLR / Prolactin Receptor Rabbit anti-Mouse Polyclonal (aa591-605) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Prolactin Receptor antibody LS-C313207 is an unconjugated rabbit polyclonal antibody to Prolactin Receptor (PRLR) (aa591-605) from mouse. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Mouse PRLR / Prolactin Receptor
Synonyms
PRLR | HPRL receptor | PRL-R | HPRLrI | Prolactin receptor
Host
Rabbit
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of mouse PRLR (591-605 aa QLGRLDYLDPTCFMH), identical to the related rat sequence, and different from the related human sequence by three amino acids.
Epitope
aa591-605
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRLR / Prolactin Receptor
P16471 NM_000949 NP_000940.1

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Featured Products

Product
PRLR / Prolactin Receptor Antibody (Extracellular Domain, clone U5) LS‑C9751
Species: Rat, Human, Pig, Rabbit
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Product
PRLR / Prolactin Receptor Antibody (Extracellular Domain, clone 1A2B1) LS‑C9755
Species: Human
Applications: Western blot, Immunoprecipitation
Product
PRLR / Prolactin Receptor Antibody (Extracellular Domain) LS‑C75083
Species: Human
Applications: IHC, Western blot, ELISA
PRLR / Prolactin Receptor Antibody - Anti-Prolactin Receptor antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ PRLR / Prolactin Receptor Antibody LS‑B2835
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
PRLR / Prolactin Receptor Antibody - PRLR / Prolactin Receptor antibody Western Blot of Lymph Node Tumor. Antibody dilution: 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PRLR / Prolactin Receptor Antibody (aa254‑303) LS‑C205742
Species: Human
Applications: Western blot
PRLR / Prolactin Receptor ELISA Kit
Human PRLR / Prolactin Receptor (Sandwich ELISA) ELISA Kit - LS-F50342
Reactivity: Human
Range: 1.46-400 ng/ml
See all PRLR / Prolactin Receptor Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
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