Sheep PRL / Prolactin
Sheep, Human, Rat, Cat, Dog
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:500 - 1:3000)
- Immunofluorescence (1:20 - 1:40)
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PRL / Prolactin antibody was raised against native sheep PRL / Prolactin.
Recognizes ovine prolactin, a 229 amino acid ~23kD somatotropin that acts primarily on the mammary gland by promoting lactation, mammogenesis, mitogenesis and osmoregulation. Prolactin can also exist in an alternative glycosylated form increasing it's apparent molecular weight to ~25kD. Glycosylation also appears to negatively affect the potency of ovine prolactin in a variety of explant bioassays. Has been successfully used for the detection of prolactin in plasma of mice by ELISA.
ELISA: In a simple ELISA, with 400 ng of antigen coated per well, the 50% OD was 1:40K. The end point titer was observed at an antibody dilution of 1:5M, under the same conditions.
PBS, 0.09% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.