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Anti-PRKDC / DNA-PKcs Antibody (phospho-Thr2609) IHC-plus™ LS-B409

Note: This antibody replaces LS-C18951
See our new PRKDC / DNA-PKcs ELISA Kits
Mouse/Human PRKDC / DNA-PKcs Cell-Based ELISA Kit - LS-F1945
Mouse PRKDC / DNA-PKcs ELISA Kit - LS-F8115
(kits do not necessarily contain the antibody listed on this page)


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)

PRKDC / DNA-PKcs Antibody LS-B409 Images

[click image for a larger image and more details]
Anti-DNA-PKcs antibody IHC of human prostate.
Western blot
Anti-DNAPKcs Antibody - Western Blot.

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Rabbit Polyclonal to Human PRKDC / DNA-PKcs
IHC - Paraffin, Western blot, ELISA


Human PRKDC / DNA-PKcs
Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


IHC - Paraffin (5 µg/ml), Western blot (1:250 - 1:2000), ELISA (1:1000 - 1:5000)

Specificity and Use

PRKDC / DNA-PKcs antibody was raised against synthetic peptide from human PRKDC / DNA-PKcs.
aa 2604-2614 of human DNA PKcs.
Immunohistochemistry: LS-B409 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B409 was determined to be 5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About PRKDC / DNA-PKcs

P78527 NM_006904 NP_008835.5

PRKDC Antibody, DNA-PK Antibody, DNAPK Antibody, DNPK1 Antibody, HYRC Antibody, HYRC1 Antibody, p350 Antibody, Scid Antibody, XRCC7 Antibody, DNA-PK catalytic subunit Antibody, DNA-PKcs Antibody, p460 Antibody

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step.


Anti-DNA-PKcs antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B409 concentration 5 ug/ml.

Western blot

Western blot
Anti-DNAPKcs Antibody - Western Blot. Western blot of Affinity Purified anti-DNAPKcs antibody shows detection of a 460 kD band corresponding to human DNAPKcs in various preparations. Lane 1: Fus1 untreated, Lane 2: Fus1 IR (20Gy, 4h), Lane 3: Fus1 DNAPK inhibitor + IR, Lane 4: MO59J (DNAPK-) untreated, Lane 5: MO59J IR, Lane 6: Fus1 untreated, Lane 7: Fus1 IR (20Gy, 4h), Lane 8: Fus1 DNAPK inhibitor + IR, Lane 9: MO59J untreated, Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1:1000 dilution of anti-DNAPKcs antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.

Requested From: United States
Date Requested: 10/7/2015

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