Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse, Rat, Hamster (tested or 100% immunogen sequence identity)
IHC - Paraffin
Western blot (1:2000)
Specificity and Use
Human DNA-PKcs fragment.
Recognizes human DNA-PKcs. Species cross-reactivity: Mouse, Rat, and hamster.
Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation and Gel Super Shift Assays. Immunohistochemistry (Paraffin): 1:100. Requires heat mediated treatment of paraffin sections. We recommend the use of antigen unmasking fluid for this purpose. A two hour incubation is recommended. Western Blot: 1:2000.
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step.