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Anti-PRKDC / DNA-PKcs Antibody (clone 1B9) IHC-plus™ LS-B5503

Note: This antibody replaces LS-C133398

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PRKDC / DNA-PKcs Antibodies

Anti-PRKDC / DNA-PKcs Antibody (phospho-Thr2609) IHC-plus™ LS-B409
Rabbit Polyclonal to Human PRKDC / DNA-PKcs
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PRKDC / DNA-PKcs Antibody (clone 42-psc, Azide-free) LS-C88135
Mouse Monoclonal [clone 42-psc] (IgG1,k) to Human PRKDC / DNA-PKcs
Human
IHC - Paraffin, Immunofluorescence, Gel shift
Unconjugated, Azide-free
Immunohistochemistry Image
Anti-PRKDC / DNA-PKcs Antibody (aa4061-4110) IHC-plus™ LS-B6857
Rabbit Polyclonal (IgG) to Human PRKDC / DNA-PKcs
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1B9] (IgG) to Human PRKDC / DNA-PKcs
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human PRKDC / DNA-PKcs
Mouse
Human (tested or 100% immunogen sequence identity)
IgG Monoclonal [1B9]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

PRKDC / DNA-PKcs antibody was raised against pRKDC (NP_008835, 4019 a.a. ~ 4129 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human DNA PKcs
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western blot using cell line lysates and the recombinant protein used as the immunogen.

Packaging

PBS, pH 7.2
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About PRKDC / DNA-PKcs

P78527 NM_006904 NP_008835.5

PRKDC Antibody, DNA-PK Antibody, DNAPK Antibody, DNPK1 Antibody, HYRC Antibody, HYRC1 Antibody, p350 Antibody, Scid Antibody, XRCC7 Antibody, DNA-PK catalytic subunit Antibody, DNA-PKcs Antibody, p460 Antibody, DNA-dependent protein kinase catalytic subunit Antibody

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step.

Immunohistochemistry

Immunohistochemistry
Anti-DNA-PKcs antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5503 concentration 5 ug/ml.

Western blot

Western blot
PRKDC monoclonal antibody clone 1B9 Western blot of PRKDC expression in A-431.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged PRKDC is 1 ng/ml as a capture antibody.
 

Requested From: United States
Date Requested: 12/4/2016

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