LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
PRKD2 / PKD2 Antibody (clone OTI5G1) LS‑C115602 Best Seller
PKD2 antibody LS-C115602 is an unconjugated mouse monoclonal antibody to PKD2 (PRKD2). Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C115602-100
100 µl (0.72 mg/ml)
Unavailable

Popular PRKD2 / PKD2 Products

Anti-PRKD2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5453 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Immunohistochemical analysis of PRKD2 (pS876) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Western blot of Phospho-PKD2 (S876) antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA

Product Description

PKD2 antibody LS-C115602 is an unconjugated mouse monoclonal antibody to PKD2 (PRKD2). Validated for Flow, IF, IHC and WB.
About PRKD2 / PKD2
Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and... Q9BZL6 NM_016457 NP_057541.2

PRKD2 Antibody, HSPC187 Antibody, NPKC-D2 Antibody, Protein kinase D2 Antibody, DKFZP586E0820 Antibody


Specifications

Target
Human PRKD2 / PKD2
Host
Mouse
Clonality
IgG2b Monoclonal [OTI5G1]
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Immunofluorescence (1:100)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PRKD2 / PKD2 antibody was raised against full length human recombinant protein of human PRKD2 (NP_057541) produced in HEK293T cell
Specificity
Human PRKD2
Usage
Concentration: 0.72 mg/ml (Lot dependent)
Presentation
PBS (PH 7.3), 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-PRKD2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKD2.
Anti-PRKD2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKD2.

Western blot

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKD2 monoclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKD2 monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKD2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKD2.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKD2 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKD2 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-PRKD2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKD2.
Anti-PRKD2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKD2.

Western blot

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKD2 monoclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKD2 monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKD2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKD2.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKD2 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKD2 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-PRKD2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKD2.
Anti-PRKD2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKD2.

Western blot

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKD2 monoclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKD2 monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKD2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKD2.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKD2 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKD2 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PRKD2 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-PRKD2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKD2.
Anti-PRKD2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKD2.

Western blot

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKD2 monoclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKD2 monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKD2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKD2.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKD2 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKD2 antibody, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/21/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number