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Anti-PRKCB / PKC-Beta Antibody (aa607-671) LS-C38534

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - Unavailable

Most Popular PRKCB / PKC-Beta Antibodies

Anti-PRKCB / PKC-Beta Antibody (N-Terminus) LS-C107572
Rabbit Polyclonal (IgG) to Human PRKCB / PKC-Beta
Human, Monkey, Mouse, Rat, Bovine, Pig
Western blot
Unconjugated
Western blot Image
Anti-PRKCB / PKC-Beta Antibody (clone Q19-V, FITC) LS-C170829
Rabbit Polyclonal [clone Q19-V] (IgG) to Human PRKCB / PKC-Beta
Human
Flow Cytometry
FITC Conjugated
Anti-PRKCB / PKC-Beta Antibody (aa631-642) IHC-plus™ LS-B9557
Goat Polyclonal to Human PRKCB / PKC-Beta
Human, Monkey, Mouse, Rat, Bovine, Horse, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human PRKCB / PKC-Beta
Human, Rat
ICC, Western blot, ELISA
Unconjugated

Details

Human PRKCB / PKC-Beta
Goat
Human, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • ICC
  • Western blot
  • ELISA

Specificity and Use

PRKCB / PKC-Beta antibody was raised against e. coli-derived rhPKCb1 (aa607-671).
aa607-671
Recognizes human and rat PKCb1. Selected for its ability to recognize PKCb1 in the applications listed.
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect PKC 1. The detection limit for rhPKC 1 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 ug/ml to detect PKC 1 in neural progenitor cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human PKC 1. The detection limit for rhPKC 1 is approximately 0.3 ng/well. In this format, this antibody shows less than 1% cross-reactivity with rhPKC.

Packaging

Lyophilized.
100 µl deionized water
For research use only.

About PRKCB / PKC-Beta

P05771 NM_002738 NP_002729.2

PRKCB Antibody, PKC beta 2 Antibody, Pkc betaII Antibody, PRKCB2 Antibody, Protein kinase c beta Antibody, Protein kinase c beta 2 Antibody, Protein kinase c beta i Antibody, Protein kinase C beta type Antibody, Protein kinase C, beta Antibody, Protein kinase C, beta 1 Antibody, PRKCB1 Antibody, Pkc betaI Antibody, PKC-beta Antibody, PKCB Antibody, Pkcbeta Antibody, PkcbetaI Antibody, PKC-B Antibody, PkcbetaII Antibody, Protein kinase c beta 1 Antibody, Protein kinase c beta II Antibody

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation.

Requested From: 
Date Requested: 3/24/2017

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