Human PRKCA / PKC-Alpha

Rabbit

Human, Rat, Rabbit (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Phospho-PKCa (Ser-657/Tyr-658) synthetic peptide (coupled to carrier protein) corresponding to amino acid residues around serine 657 and tyrosine 658 of human PKCa. This sequence is similar to a dual phosphorylation site found in PKCbI (Ser-661/Tyr-662). These human sequences are highly conserved in rat and mouse PKCa and PKCbI. In addition, this dual site is homologous to threonine 674 and tyrosine 675 in human PKCg, although this reactivity has not been confirmed.

pSer657/Tyr658

Detects an 82kD protein corresponding to the molecular mass of phosphorylated PKCa on SDS-PAGE immunoblots of neonatal rat brain lysate. Similar results were seen in other PKCa-expressing cells, such as human SKN-SH, endothelial, and HeLa cells, as well as rabbit spleen fibroblasts and rat pituitary cells. In immunoprecipitation experiments with various PKC isoforms, this antibody detected PKCa and PKCb, but not other PKC isoforms in rat brain lysate.

• Western blot
• ELISA

Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols.

PBS, 1 mg/ml BSA, 0.05% Sodium Azide, 50% Glycerol

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P17252 NCBI: NM_002737 NP_002728.1