Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Human PRKCA / PKC-Alpha
IHC - Paraffin, Western blot
Rabbit Polyclonal to Human PRKCA / PKC-Alpha
Human, Monkey, Mouse, Rat
ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Human PRKCA / PKC-Alpha
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Western blot (1:1000)
Flow Cytometry (1:25)
Specificity and Use
Synthetic peptide (KLH-coupled) corresponding to human PKCa.
Detects endogenous levels of total PKCa protein. Does not cross-react with endogenous levels of other PKC isoform MW: 80kD. Species cross-reactivity: Monkey, Mouse and Rat.
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Flow Cytometry: 1:25. Western Blot: 1:1000. Immunoprecipitation: 1:50. Immunocytochemistry: 1:50. Immunofluorescence: 1:50.
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100 ug/ml BSA, 50% glycerol.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
PRKCA Antibody, Aging-associated gene 6 Antibody, AAG6 Antibody, Alpha PKC Antibody, PKC-A Antibody, Pkcalpha Antibody, Protein kinase C alpha type Antibody, PKC Alpha Antibody, PKC-alpha Antibody, PKCA Antibody, Protein kinase c alpha Antibody, Protein kinase C, alpha Antibody
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP.