Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse (tested or 100% immunogen sequence identity)
Western blot (0.1 - 0.75 µg/ml)
ELISA (0.1 - 0.75 µg/ml)
Specificity and Use
PRKACA + PRKACB antibody was raised against synthetic phosphopeptide derived from a region of human PKA that contains threonine 197. The sequence is conserved among many species for PKAa, b, and g, including mouse, rat, bovine, porcine and frog.
Recognizes mouse PKA catalytic subunits a and b. Other species (100% homologous) have not been tested, but are expected to react. This antibody exibited a preference for PKA catalytic subunit b in some cell lines tested.
Suitable for use in Western Blot and ELISA. Western Blot: 0.1-0.75 ug/ml. At 0.35 ug/ml, the dilution provides 100 ml working solution, which at 10 ml/blot allows 10 blots to be performed. Positive control: Forskolin-treated NIH3T3 cells, or Y-1 mouse adrenal cortical cells.