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Anti-PRKACA + PRKACB Antibody (aa1-351) LS-C185887

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml Unavailable

Most Popular PRKACA + PRKACB Antibodies

Anti-PRKACA + PRKACB Antibody (aa9-40) LS-C98916
Rabbit Polyclonal to Human PRKACA + PRKACB
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PRKACA + PRKACB Antibody (aa67-94) LS-C162147
Rabbit Polyclonal to Human PRKACA + PRKACB
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-PRKACA + PRKACB Antibody (phospho-Thr197) LS-C199444
Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

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Rabbit Polyclonal (IgG) to Human PRKACA + PRKACB
Human
ICC, Western blot
Unconjugated

Details

Human PRKACA + PRKACB
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

PRKACA + PRKACB antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 351 of PKA C alpha (SwissProt P17612).
aa1-351
Human PRKACA

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About PRKACA + PRKACB

P17612 NM_002730 NP_002721.1

PRKACA Antibody, PKA C-alpha Antibody, Pka calpha Antibody, PKACA Antibody

Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation.

Immunocytochemistry

Immunocytochemistry
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using PKA alpha antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: NIH-3T3, B: C2C12. 10% SDS PAGE. PRKACA antibody diluted at 1:1000.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: HeLa S3. 12% SDS PAGE. PRKACA antibody diluted at 1:500
 

Requested From: 
Date Requested: 3/30/2017

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