Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation.
Immunofluorescence of monoclonal antibody to PRKAA2 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of PRKAA2 expression in transfected 293T cell line by PRKAA2 monoclonal antibody (M02), clone 1G8.Lane 1: PRKAA2 transfected lysate(62.3 KDa).Lane 2: Non-transfected lysate.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged PRKAA2 is approximately 0.03 ng/ml as a capture antibody.
Western blot analysis of PRKAA2 over-expressed 293 cell line, cotransfected with PRKAA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKAA2 monoclonal antibody (M02) clone 1G8 . GAPDH ( 36.1 kDa ) used as specificity and loading control.