Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 8E11H5] (IgG1) to Human PRKAA2 / AMPK Alpha 2
IHC, Western blot, Flow Cytometry, ELISA
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Human PRKAA2 / AMPK Alpha 2
Human (tested or 100% immunogen sequence identity)
Rat, Bovine, Pig, Chicken, Xenopus (at least 90% immunogen sequence identity)
IHC - Paraffin (1:100 - 1:1000)
ICC (1:100 - 1:1000)
Western blot (1:500 - 1:3000)
Immunoprecipitation (1:100 - 1:500)
Specificity and Use
Recombinant fragment corresponding to a region within amino acids 29 and 510 of AMPK alpha 2 (SwissProt P54646). Percent identity by BLAST analysis: Human (100%); Pig, Bovine (99%); Rat (98%); Chicken, Xenopus (96%).
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation.
IHC of paraffin-embedded A549 xenograft using AMPK alpha 2 antibody at 1:500 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using AMPK alpha 2 antibody at 1:200 dilution.
Sample (30 ug whole cell lysate). A: A431, B: MOLT4 . 7.5% SDS PAGE. PRKAA2 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. PRKAA2 antibody diluted at 1:1000.
AMPK alpha 2 antibody immunoprecipitates AMPK alpha 2 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of AMPK alpha 2 protein by 2.5 ug of AMPK alpha 2 antibody 10% SDS-PAGE The immunoprecipitated AMPK alpha 2 protein was detected by AMPK alpha 2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.