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Anti-PRKAA2 / AMPK Alpha 2 Antibody (aa29-510) LS-C185542


Wt. Vol. Conc. Price
- 100 µl 0.55 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular PRKAA2 / AMPK Alpha 2 Antibodies

Anti-PRKAA2 / AMPK Alpha 2 Antibody (phospho-Ser175) LS-C46806
Rabbit Polyclonal to Human PRKAA2 / AMPK Alpha 2
Western blot, ELISA
Anti-PRKAA2 / AMPK Alpha 2 Antibody (C-Terminus, AP) LS-C218629
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
IHC, Western blot, ELISA
AP Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human PRKAA2 / AMPK Alpha 2
IHC - Paraffin, ICC, Western blot, Immunoprecipitation


Human PRKAA2 / AMPK Alpha 2
Human (tested or 100% immunogen sequence identity)
Rat, Bovine, Pig, Chicken, Xenopus (at least 90% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
  • Immunoprecipitation (1:100 - 1:500)

Specificity and Use

PRKAA2 / AMPK Alpha 2 antibody was raised against recombinant fragment corresponding to a region within amino acids 29 and 510 of AMPK alpha 2 (SwissProt P54646). Percent identity by BLAST analysis: Human (100%); Pig, Bovine (99%); Rat (98%); Chicken, Xenopus (96%).
Human PRKAA2
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).


0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About PRKAA2 / AMPK Alpha 2

P54646 NM_006252 NP_006243.2

PRKAA2 Antibody, ACACA kinase Antibody, Alpha2 AMPK Antibody, AMPK alpha 2 Antibody, AMPK subunit alpha-2 Antibody, Acetyl-CoA carboxylase kinase Antibody, AMPK-2 Antibody, AMPKa2 Antibody, AMPKalpha2 Antibody, Ampk alpha2 Antibody, AMPK2 Antibody, PRKAA Antibody, AMPK Antibody, AMPK-alpha-2 chain Antibody, HMGCR kinase Antibody

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation.


IHC of paraffin-embedded A549 xenograft using AMPK alpha 2 antibody at 1:500 dilution.


Immunofluorescence of paraformaldehyde-fixed A431 using AMPK alpha 2 antibody at 1:200 dilution.

Western blot

Western blot
Sample (30 ug whole cell lysate). A: A431, B: MOLT4 . 7.5% SDS PAGE. PRKAA2 antibody diluted at 1:1000

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. PRKAA2 antibody diluted at 1:1000.


AMPK alpha 2 antibody immunoprecipitates AMPK alpha 2 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of AMPK alpha 2 protein by 2.5 ug of AMPK alpha 2 antibody 10% SDS-PAGE The immunoprecipitated AMPK alpha 2 protein was detected by AMPK alpha 2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Requested From: United States
Date Requested: 10/25/2016

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