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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PRKAA1 / AMPK Alpha 1 Antibody (aa376‑392) LS‑C11841
AMPK Alpha 1 antibody LS-C11841 is an unconjugated rabbit polyclonal antibody to AMPK Alpha 1 (PRKAA1) from human, mouse, rat and other species. Validated for ELISA and WB.
Antibody
Catalog
Size
Price
LS-C11841-200
200 µg
$605

Popular PRKAA1 / AMPK Alpha 1 Products

Antibody
Species: Human, Monkey, Mouse, Rat, Hamster
Applications: Western blot
Antibody
Species: Drosophila
Applications: IHC, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
IHC of paraffin-embedded ovarian cancer (left) and brain tissues (right) using PRKAA1 mouse monoclonal antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Immunohistochemical analysis of AMPK alpha 1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Chicken
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
IHC of AMPK1/AMPK2 (T479) pAb in paraffin-embedded human colon carcinoma tissue.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

AMPK Alpha 1 antibody LS-C11841 is an unconjugated rabbit polyclonal antibody to AMPK Alpha 1 (PRKAA1) from human, mouse, rat and other species. Validated for ELISA and WB.

Specifications

Target
Human PRKAA1 / AMPK Alpha 1
Synonyms
PRKAA1, ACACA kinase, AMPK alpha 1, AMPK alpha-1 chain, Acetyl-CoA carboxylase kinase, AMPKa1, AMPK subunit alpha-1, AMPK, AMPK1, Tau-protein kinase PRKAA1, HMGCR kinase
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
Predicted
Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
  • ELISA
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide of human AMP-activated Protein Kinase a1 corresponding to aa 376-392 (C-ARHTLDELNPQKSKHQG-COOH) (KLH coupled). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Horse, Pig (100%); Opossum, Turkey, Chicken, Platypus (94%); Xenopus, Zebrafish (88%); Pufferfish (82%).
Epitope
aa376-392
Specificity
Recognizes human AMPK a1, Mr 63kD. Species cross-reactivity: rat and mouse.
Usage
Suitable for use in ELISA and Western Blot. Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About PRKAA1 / AMPK Alpha 1
Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. Q13131 NM_006251 NP_006242.5


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Requested From: United States
Date Requested: 10/21/2018

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