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Anti-PRDM5 Antibody (aa36-85) IHC-plus™ LS-B3500

Note: This antibody replaces LS-C101415

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PRDM5 Antibodies

Anti-PRDM5 Antibody (aa100-200) IHC-plus™ LS-B1982
Rabbit Polyclonal to Human PRDM5
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PRDM5 Antibody (aa36-85) IHC-plus™ LS-B3500
Rabbit Polyclonal (IgG) to Human PRDM5
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PRDM5 Antibody LS-C349088
Rabbit Polyclonal (IgG) to Human PRDM5
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human PRDM5
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit, Zebrafish
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human PRDM5
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Dog, Chicken (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

PRDM5 antibody was raised against synthetic peptide located between aa36-85 of human PRDM5 (Q9NQX1, NP_061169). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Rabbit, Horse, Pig, Guinea pig (100%); Dog, Turkey, Zebra finch, Chicken (92%); Bat (85%).
LS-E11558 - Lyophilized - 100 µg - $145.00
aa36-85
Human PRDM5
Immunohistochemistry: LS-B3500 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3500 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About PRDM5

Q9NQX1 NM_018699 NP_061169.2

PRDM5 Antibody, BCS2 Antibody, PFM2 Antibody, PR domain containing 5 Antibody, PR domain-containing protein 5 Antibody

Sequence-specific DNA-binding transcription factor. Represses transcription at least in part by recruitment of the histone methyltransferase EHMT2/G9A and histone deacetylases such as HDAC1. Regulates hematopoiesis-associated protein-coding and microRNA (miRNA) genes.

Immunohistochemistry

Immunohistochemistry
Anti-PRDM5 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3500 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
PRDM5 antibody LS-B3500 Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/10/2016

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