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Anti-PRDM2 / RIZ1 Antibody (clone 33AT1045) LS-C96458

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PRDM2 / RIZ1 Antibodies

Anti-PRDM2 / RIZ1 Antibody LS-C98738
Rabbit Polyclonal to Rat PRDM2 / RIZ1
Rat, Human, Mouse
IHC
Unconjugated
Immunohistochemistry Image
Anti-PRDM2 / RIZ1 Antibody (aa1-347, clone 33AT1045, Azide-free, HRP) LS-C250394
Mouse Monoclonal [clone 33AT1045] (IgG1) to Human PRDM2 / RIZ1
Human, Mouse
Western blot, Immunoprecipitation, ELISA
HRP Conjugated, Azide-free
Anti-PRDM2 / RIZ1 Antibody (aa245-573, APC) LS-C267064
Rabbit Polyclonal (IgG) to Human PRDM2 / RIZ1
Human, Mouse, Rat
IHC, Western blot, Immunoprecipitation, ELISA
APC Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 33AT1045] (IgG1) to Human PRDM2 / RIZ1
Human, Mouse
Western blot, Chromatin Immunoprecipitation
Unconjugated

Details

Human PRDM2 / RIZ1
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [33AT1045]
Unconjugated
Protein G purified
Unmodified

Applications

  • Western blot (1:1000)
  • Chromatin Immunoprecipitation (1:100)

Specificity and Use

PRDM2 / RIZ1 antibody was raised against purified recombinant GST fusion protein encoding aa 1-347 of human RIZ1. This antibody is specific for RIZ1, it does not recognize the isoform beta (RIZ2).

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About PRDM2 / RIZ1

Q13029 NM_012231 NP_036363.2

PRDM2 Antibody, GATA-3 binding protein G3B Antibody, GATA-3-binding protein G3B Antibody, HUMHOXY1 Antibody, KMT8 Antibody, Lysine N-methyltransferase 8 Antibody, RIZ2 Antibody, MTE-binding protein Antibody, PR domain-containing protein 2 Antibody, RIZ Antibody, Zinc finger protein RIZ Antibody, MTB-ZF Antibody, RIZ1 Antibody

This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene.

Western blot

Western blot
Western blot of anti-PRDM2 antibody in lysate from PRDM2 transgenic lysate.Secondary HRP-anti-mouse was used for signal visualization with chemiluminescence.

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation
ChIP of estrogen target genes. (C) ChIP analysis was performed on RIZ1 knockout mouse embryonic fibroblasts by using anti-RIZ1 antibody. Immunoprecipitated chromatin DNA was analyzed by PCR with primers in the G6pd promoter region and in the cdc25c promoter region. Adapted from Fig 6 in Carling et al., 2004 (see citation 2).

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation
ChIP of estrogen target genes.(B) Time course of RIZ1 binding to the pS2 gene promoter. MCF7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis. Immunoprecipitation was performed with antibody to RIZ1 and control immunoglobulin G as indicated. Adapted from Fig 6 in Carling et al., 2004 (see citation 2).

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation
ChIP of estrogen target genes. (A) Soluble chromatin was prepared from MCF7 cells not treated or treated with E2 for 45 min. Immunoprecipitation was performed with antibody against RIZ1. DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter. Adapted from Fig 6 in Carling et al., 2004 (see citation 2).

Requested From: United States
Date Requested: 12/11/2016

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