Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
PRDM2 Antibody, GATA-3 binding protein G3B Antibody, GATA-3-binding protein G3B Antibody, HUMHOXY1 Antibody, KMT8 Antibody, Lysine N-methyltransferase 8 Antibody, RIZ2 Antibody, MTE-binding protein Antibody, PR domain-containing protein 2 Antibody, RIZ Antibody, Zinc finger protein RIZ Antibody, MTB-ZF Antibody, RIZ1 Antibody
This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene.
Western blot of anti-PRDM2 antibody in lysate from PRDM2 transgenic lysate.Secondary HRP-anti-mouse was used for signal visualization with chemiluminescence.
ChIP of estrogen target genes. (C) ChIP analysis was performed on RIZ1 knockout mouse embryonic fibroblasts by using anti-RIZ1 antibody. Immunoprecipitated chromatin DNA was analyzed by PCR with primers in the G6pd promoter region and in the cdc25c promoter region. Adapted from Fig 6 in Carling et al., 2004 (see citation 2).
ChIP of estrogen target genes.(B) Time course of RIZ1 binding to the pS2 gene promoter. MCF7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis. Immunoprecipitation was performed with antibody to RIZ1 and control immunoglobulin G as indicated. Adapted from Fig 6 in Carling et al., 2004 (see citation 2).
ChIP of estrogen target genes. (A) Soluble chromatin was prepared from MCF7 cells not treated or treated with E2 for 45 min. Immunoprecipitation was performed with antibody against RIZ1. DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter. Adapted from Fig 6 in Carling et al., 2004 (see citation 2).