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Anti-PRDM14 Antibody LS-C150037

Ordering

Wt. Vol. Conc. Price
100 µg - - Unavailable

Most Popular PRDM14 Antibodies

Anti-PRDM14 Antibody (aa208-257) LS-C31290
Rabbit Polyclonal (IgG) to Human PRDM14
Human, Bovine, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PRDM14 Antibody (aa1-30) LS-C98753
Rabbit Polyclonal to Human PRDM14
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PRDM14 Antibody (clone 647002) LS-C150036
Mouse Monoclonal [clone 647002] (IgG1) to Human PRDM14
Human
ICC, Western blot
Unconjugated
Immunocytochemistry Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human PRDM14
Human
ICC, Western blot, ELISA
Unconjugated

Details

Human PRDM14
Sheep
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (10 µg/ml)
  • Western blot (0.1 µg/ml)
  • ELISA (0.5 - 1 µg/ml)

Specificity and Use

PRDM14 antibody was raised against e. coli-derived rhPRDM14.
Human PRDM14.
Western blot-An antibody concentration of 0.1 µg/ml is recommended. Immunocytochemistry-This antibody has been used at a concentration of 10 µg/ml to detect PRDM14 in BG0IV cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1 % Triton X-100 and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody for 3 hours at room temperature followed by donkey anti-sheep IgG at room temperature for 1 hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA-This antibody can be used at 0.5-1.0 µg/ml with the appropriate secondary reagents to detect human PRDM14. The detection limit for rhPRDM14 is approximately 0.1 ng/well.

Packaging

Lyophilized from 0.2 µm filtered 100 mM NaHCO3, pH 8.3, 500 mM NaCl, 1% sucrose.
Store at -20°C.
For research use only.

About PRDM14

Q9GZV8 NM_024504 NP_078780.1

PRDM14 Antibody, PFM11 Antibody, PR domain containing 14 Antibody

Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression (By similarity).

Western blot

Western blot
Detection of PRDM14. Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 0.1 ug/ml sheep anti-hPRDM14.
 

Requested From: 
Date Requested: 3/30/2017

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