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Anti-PRDM14 Antibody (clone 647002) LS-C150036

Ordering

Wt. Vol. Conc. Price
100 µg - - $315
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PRDM14 Antibodies

Anti-PRDM14 Antibody (clone 647002) LS-C150036
Mouse Monoclonal [clone 647002] (IgG1) to Human PRDM14
Human
ICC, Western blot
Unconjugated
Immunocytochemistry Image
Anti-PRDM14 Antibody (aa1-30, AP) LS-C239403
Rabbit Polyclonal (IgG) to Human PRDM14
Human
IHC, ICC, Immunofluorescence, Western blot, ELISA
AP Conjugated
Anti-PRDM14 Antibody (aa1-30, FITC) LS-C239406
Rabbit Polyclonal (IgG) to Human PRDM14
Human
IHC, ICC, Immunofluorescence, Western blot, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 647002] (IgG1) to Human PRDM14
Human
ICC, Western blot
Unconjugated

Details

Human PRDM14
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [647002]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • ICC (8 - 25 µg/ml)
  • Western blot (2 µg/ml)

Specificity and Use

PRDM14 antibody was raised against e. coli-derived recombinant human PRDM14 Ala2-Asp201 Accession # Q9-GZV8.
Detects human PRDM14 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse PRDM14.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 200 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About PRDM14

Q9GZV8 NM_024504 NP_078780.1

PRDM14 Antibody, PFM11 Antibody, PR domain containing 14 Antibody

Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression (By similarity).

Immunocytochemistry

Immunocytochemistry
PRDM14 in BG01 V Human Cell Line. PRDM14 was detected in fixed BG01 V human embryonic stem cells grown on irradiated MEF cells using Human PRDM14 Monoclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.

Western blot

Western blot
Detection of Human PRDM14 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 2 ug/ml of Human PRDM14 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for PRDM14 at approximately 80 kD (as indicated). This experiment was conducted under reducing conditions.

Requested From: United States
Date Requested: 12/7/2016

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