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Anti-PRAME Antibody (aa71-120) LS-C111766

Ordering

Wt. Vol. Conc. Price
50 µg - - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PRAME Antibodies

Anti-PRAME Antibody LS-C47362
Rabbit Polyclonal to Human PRAME
Human, Monkey, Bovine, Dog, Horse
Western blot
Unconjugated
Western blot Image
Anti-PRAME Antibody (aa476-502) LS-C164013
Rabbit Polyclonal to Human PRAME
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-PRAME Antibody IHC-plus™ LS-B10827
Rabbit Polyclonal (IgG) to Human PRAME
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human PRAME
Human, Horse, Pig, Rabbit
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human PRAME
Rabbit
Human, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Bovine (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

PRAME antibody was raised against synthetic peptide located between aa71-120 of human PRAME (P78395, NP_006106). Percent identity by BLAST analysis: Human, Gibbon, Monkey (100%); Gorilla, Mouse, Rat, Bovine (92%); Marmoset (91%); Hamster, Pig (84%).
LS-E20272 - Lyophilized - 100 µg - $145.00
aa71-120
Human PRAME
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About PRAME

P78395 NM_006115 NP_006106.1

PRAME Antibody, Cancer/testis antigen 130 Antibody, CT130 Antibody, MAPE Antibody, Opa-interacting protein 4 Antibody, Opa-interacting protein OIP4 Antibody, OIP-4 Antibody, OIP4 Antibody

PRAME encodes a 509 amino acid antigen, lacking a signal sequence, and recognized on a human melanoma cell line by a T-lymphocyte clone. A significant level of PRAME mRNA is detected in normal testis as well as in many melanomas, non-small cell lung carcinomas, sarcomas, head and neck tumors and renal carcinomas. PRAME is expressed predominantly in acute leukemias carrying chromosomal abnormalities such as translocation t(8:21), which fuses the AML1 and ETO genes.

Western blot

Western blot
PRAME antibody LS-C111766 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/10/2016

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