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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PPP5C Antibody (clone 3G1) LS‑C114694
PPP5C antibody LS-C114694 is an unconjugated mouse monoclonal antibody to PPP5C. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C114694-100
100 µl
Unavailable

Popular PPP5C Products

Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-PPP5C mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded thyroid tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Species: Human, Dog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded pancreas tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

PPP5C antibody LS-C114694 is an unconjugated mouse monoclonal antibody to PPP5C. Validated for Flow, IF, IHC and WB.
About PPP5C
Serine/threonine-protein phosphatase that dephosphorylates a myriad of proteins involved in different signaling pathways including the kinases CSNK1E, ASK1/MAP3K5, PRKDC and RAF1, the nuclear receptors NR3C1, PPARG, ESR1 and ESR2, SMAD proteins and TAU/MAPT. P53041 NM_006247 NP_006238.1

PPP5C Antibody, PP-T Antibody, Protein phosphatase T Antibody, PPP5 Antibody, PPT Antibody, Protein phosphatase 5 Antibody, PP5 Antibody


Specifications

Target
Human PPP5C
Host
Mouse
Clonality
IgG2b Monoclonal [3G1]
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PPP5C antibody was raised against protein expressed in 293T cell transfected with human PPP5C expression vector
Specificity
Human PPP5C
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded prostate tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded colon tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded colon tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-PPP5C mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP5C.
Anti-PPP5C mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP5C.

Immunofluorescence

Immunofluorescent staining of HeLa cells using anti-PPP5C mouse monoclonal antibody.
Immunofluorescent staining of HeLa cells using anti-PPP5C mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP5C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP5C.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP5C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP5C.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY PPP5C (Red) or empty vector control plasmid (Blue) were immunostained with anti-PPP5C mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY PPP5C (Red) or empty vector control plasmid (Blue) were immunostained with anti-PPP5C mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded prostate tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded colon tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded colon tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-PPP5C mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP5C.
Anti-PPP5C mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP5C.

Immunofluorescence

Immunofluorescent staining of HeLa cells using anti-PPP5C mouse monoclonal antibody.
Immunofluorescent staining of HeLa cells using anti-PPP5C mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP5C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP5C.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP5C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP5C.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY PPP5C (Red) or empty vector control plasmid (Blue) were immunostained with anti-PPP5C mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY PPP5C (Red) or empty vector control plasmid (Blue) were immunostained with anti-PPP5C mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded prostate tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded colon tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded colon tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-PPP5C mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP5C.
Anti-PPP5C mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP5C.

Immunofluorescence

Immunofluorescent staining of HeLa cells using anti-PPP5C mouse monoclonal antibody.
Immunofluorescent staining of HeLa cells using anti-PPP5C mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP5C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP5C.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP5C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP5C.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY PPP5C (Red) or empty vector control plasmid (Blue) were immunostained with anti-PPP5C mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY PPP5C (Red) or empty vector control plasmid (Blue) were immunostained with anti-PPP5C mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded prostate tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded colon tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded colon tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-PPP5C mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-PPP5C mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP5C.
Anti-PPP5C mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP5C.

Immunofluorescence

Immunofluorescent staining of HeLa cells using anti-PPP5C mouse monoclonal antibody.
Immunofluorescent staining of HeLa cells using anti-PPP5C mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP5C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP5C.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP5C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP5C.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY PPP5C (Red) or empty vector control plasmid (Blue) were immunostained with anti-PPP5C mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY PPP5C (Red) or empty vector control plasmid (Blue) were immunostained with anti-PPP5C mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/19/2018

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