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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PPP2R3A / PR130 Antibody (aa1073‑1085) IHC‑plus™ LS‑B2589 Best Seller
Note: This antibody replaces LS-C61780
PR130 antibody LS-B2589 is an unconjugated goat polyclonal antibody to PR130 (PPP2R3A) from human, mouse, horse and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2589-50
50 µg (0.5 mg/ml)
Unavailable

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Product Description

PR130 antibody LS-B2589 is an unconjugated goat polyclonal antibody to PR130 (PPP2R3A) from human, mouse, horse and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About PPP2R3A / PR130
PPP2R3A / PR130 is one of the regulatory subunits of the protein phosphatase 2. Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. Q06190 NM_002718 NP_002709.2

PPP2R3A Antibody, PR130 Antibody, PR72 Antibody, PPP2R3 Antibody, PP2A, subunit B, R3 isoform Antibody


Specifications

Target
Human PPP2R3A / PR130
Host
Goat
Reactivity
Human, Monkey, Mouse, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Rat, Bat, Bovine, Hamster, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PPP2R3A / PR130 antibody was raised against synthetic peptide C-QKDVENDGPEPSD from an internal region of human PPP2R3A (NP_002709.2; NP_871626.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Horse, Rabbit, Pig, Lizard (100%); Rat, Hamster, Bat, Bovine, Opossum, Turkey, Chicken (92%); Dog (85%).
Blocking Peptide
LS-E26555 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2589. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1073-1085
Specificity
Human PPP2R3A. This antibody is expected to recognise both reported isoforms (NP_002709.2; NP_871626.1).
Usage
Immunohistochemistry: LS-B2589 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2589 was determined to be 5 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-PPP2R3A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2589 concentration 5 ug/ml.
Anti-PPP2R3A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2589 concentration 5 ug/ml.

Immunohistochemistry

Anti-PPP2R3A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2589 concentration 5 ug/ml.
Anti-PPP2R3A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2589 concentration 5 ug/ml.

Immunohistochemistry

Anti-PPP2R3A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2589 concentration 5 ug/ml.
Anti-PPP2R3A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2589 concentration 5 ug/ml.

Immunohistochemistry

Anti-PPP2R3A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2589 concentration 5 ug/ml.
Anti-PPP2R3A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2589 concentration 5 ug/ml.

Requested From: 
Date Requested: 7/15/2018

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