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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PPP1R8 / Rnase E Antibody (aa215‑264) LS‑C101840
Rnase E antibody LS-C101840 is an unconjugated rabbit polyclonal antibody to Rnase E (PPP1R8) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C101840-50
50 µg
Unavailable

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Product Description

Rnase E antibody LS-C101840 is an unconjugated rabbit polyclonal antibody to Rnase E (PPP1R8) from human, mouse, rat and other species. Validated for WB.
About PPP1R8 / Rnase E
Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. Q12972 NM_002713 NP_002704.1

PPP1R8 Antibody, ARD-1 Antibody, ARD1 Antibody, Activator of RNA decay Antibody, NIPP-1 Antibody, NIPP1 Antibody, Nuclear subunit of PP-1 Antibody, PRO2047 Antibody, RNase E Antibody


Specifications

Target
Human PPP1R8 / Rnase E
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)
Predicted
Bat, Guinea pig, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa215-264 of human PPP1R8 (Q12972, NP_054829). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog, Bovine, Horse (100%); Elephant, Bat, Rabbit, Opossum, Guinea pig (92%); Mouse, Rat, Hamster (85%).
Blocking Peptide
LS-E14731 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101840. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa215-264
Specificity
Human PPP1R8
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

PPP1R8 antibody LS-C101840 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PPP1R8 antibody LS-C101840 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PPP1R8 antibody LS-C101840 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PPP1R8 antibody LS-C101840 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PPP1R8 antibody LS-C101840 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PPP1R8 antibody LS-C101840 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PPP1R8 antibody LS-C101840 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PPP1R8 antibody LS-C101840 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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