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Anti-PPP1R1B / DARPP-32 Antibody (N-Terminus) LS-C42472

Ordering

Wt. Vol. Conc. Price
- 100 µl - $420
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PPP1R1B / DARPP-32 Antibodies

Anti-PPP1R1B / DARPP-32 Antibody LS-C22756
Rabbit Polyclonal (IgG) to Human PPP1R1B / DARPP-32
Human, Mouse, Rat
Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (aa51-204) LS-C36138
Rat Monoclonal (IgG2a) to Human PPP1R1B / DARPP-32
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody LS-C150127
Goat Polyclonal (IgG) to Human PPP1R1B / DARPP-32
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

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Rabbit Polyclonal (IgG) to Rat PPP1R1B / DARPP-32
Rat
Western blot
Unconjugated

Details

Rat PPP1R1B / DARPP-32
Rabbit
Rat (tested or 100% immunogen sequence identity)
Human, Mouse, Bovine, Dog, Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Purified
Unmodified

Applications

Western blot (1:1000)

Specificity and Use

PPP1R1B / DARPP-32 antibody was raised against peptide corresponding to the N-terminal region of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH). ( N-TERMINAL ).
N-Terminus
Recognizes rat Protein phosphatase 1 regulatory subunit 1B, also known as DARPP-32 or Dopamine- and cAMP-regulated neuronal phosphoprotein. DARPP-32 is a 205 amino acid ~32 kD member of the protein phosphatase inhibitor 1 family. Originally discovered as a substrate for cAMP-dependent protein kinase which modulates cell signalling in neurons. DARPP-32 is converted into an inhibitor of protein phosphatase-1 when phosphorylated by protein kinase A at threonine 34, as a result of dopaminergic receptor stimulation. However this phosphorylation is reversed on stimulation of N-methyl-D-aspartate (NMDA) receptors by glutamate. Conversely when phosphorylated at threonine-75 by CDK5, DARPP-32 becomes an inhibitor of protein kinase A. DARPP-32 is highly expressed in striatonigral and striatopallidal projection neurons but is not observed in cholinergic and neuropeptide Y-containing striatal interneurons. A truncated form of DARPP-32, t-DARPP is over-expressed in gastric cancer cells and, further t-DARPP has subsequently been identified as overexpressed in Her-2 positive breast cancer cells and implicated in resistance of these cells to Herceptin treatment. DARPP-32 appears to have evolved early in vertebrate evolution and may have first appeared in gnathostomes. The immunogen sequence foris identical in canine, chicken, human, mouse and Xenopus, therefore the antibody therefore is expected to recognize DARP-32 in all these species. Is not expected to recognize t-DARPP as this lacks the amino sequence used for immunization. For the identification of specific phospho DARPP-32 forms the use af antibodies against the phospho-epitopes is recommended.
Western Blot: LS-C42472 detects a band of approximately 32kD in rat hippocampal lysate.

Packaging

10 mM HEPES, pH 7.5, 0.09% sodium azide, 0.01% BSA, 50% glycerol.
For research use only.

About PPP1R1B / DARPP-32

Q9UD71 NM_032192 NP_115568.2

PPP1R1B Antibody, DARPP-32 Antibody, DARPP32 Antibody

PPP1R1B / DARPP-32 is a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene.

Requested From: United States
Date Requested: 12/9/2016

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