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Anti-PPP1R1B / DARPP-32 Antibody LS-C150127

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PPP1R1B / DARPP-32 Antibodies

Anti-PPP1R1B / DARPP-32 Antibody LS-C22756
Rabbit Polyclonal (IgG) to Human PPP1R1B / DARPP-32
Human, Mouse, Rat
Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody (aa51-204) LS-C36138
Rat Monoclonal (IgG2a) to Human PPP1R1B / DARPP-32
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-PPP1R1B / DARPP-32 Antibody LS-C150127
Goat Polyclonal (IgG) to Human PPP1R1B / DARPP-32
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

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Goat Polyclonal (IgG) to Human PPP1R1B / DARPP-32
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated

Details

Human PPP1R1B / DARPP-32
Goat
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

PPP1R1B / DARPP-32 antibody was raised against e. coli-derived recombinant human DARPP-32 Arg51-Ala204 Accession # Q94D71.
Detects human, mouse, and rat DARPP-32 in Western blots.

Packaging

Lyophilized from 0.2 um sterile-filtered solution in PBS, 5% trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About PPP1R1B / DARPP-32

Q9UD71 NM_032192 NP_115568.2

PPP1R1B Antibody, DARPP-32 Antibody, DARPP32 Antibody

PPP1R1B / DARPP-32 is a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunohistochemistry

Immunohistochemistry
DARPP32 in Rat Brain. DARPP32 was detected in perfusion fixed frozen sections of rat brain (globus pallidus) using Goat Anti-Human/Mouse/Rat DARPP32 Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4 °C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes.

Immunohistochemistry

Immunohistochemistry
DARPP32 in Mouse Brain. DARPP32 was detected in perfusion fixed frozen sections of mouse brain (caudate putamen) using Goat Anti-Human/Mouse/Rat DARPP32 Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4 °C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes.

Immunohistochemistry

Immunohistochemistry
DARPP32 in Human Brain. DARPP32 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human/Mouse/Rat DARPP32 Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4 °C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and glial cells.

Western blot

Western blot
Detection of Human, Mouse, and Rat DARPP32 by Western Blot. Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF Membrane was probed with 1 ug/ml of Goat Anti-Human/Mouse/Rat DARPP32 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for DARPP32 at approximately 32-35 kD (as indicated).

Requested From: United States
Date Requested: 12/9/2016

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