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Anti-PPP1R15A / GADD34 Antibody (aa664-674) LS-C55303

Note: This antibody replaces LS-C63403
Catalog Size Price
LS-C55303-100 100 µg Unavailable

Most Popular PPP1R15A / GADD34 Antibodies

Anti-PPP1R15A / GADD34 Antibody (aa116-165) LS-C146499
Rabbit Polyclonal to Human PPP1R15A / GADD34
Human, Monkey
Western blot
Unconjugated
PPP1R15A / GADD34 antibody LS-C146499 Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PPP1R15A / GADD34 Antibody (clone 3D12) LS-C173753
Mouse Monoclonal [clone 3D12] (IgG2b) to Human PPP1R15A / GADD34
Human, Monkey, Mouse, Rat, Dog
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
IHC of paraffin-embedded Human tonsil using anti-PPP1R15A mouse monoclonal antibody.
Anti-PPP1R15A / GADD34 Antibody (clone 1E1) LS-C173778
Mouse Monoclonal [clone 1E1] (IgG1) to Human PPP1R15A / GADD34
Human
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PPP1R15A mouse monoclonal antibody.
Anti-PPP1R15A / GADD34 Antibody (aa433-636, Biotin) LS-C686856
Rabbit Polyclonal (IgG) to Mouse PPP1R15A / GADD34
Mouse
IHC, Western blot
Biotin Conjugated
Western blot of PPP1R15A / GADD34 antibody.
Anti-PPP1R15A / GADD34 Antibody (aa335-578, Biotin) LS-C686858
Rabbit Polyclonal (IgG) to Rat PPP1R15A / GADD34
Rat
IHC, Western blot
Biotin Conjugated
Western blot of PPP1R15A / GADD34 antibody.

100% Guaranteed
Goat Polyclonal to Human PPP1R15A / GADD34
Human
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated

Details

Human PPP1R15A / GADD34
Goat
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:128000) & (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)

Specificity and Use

Synthetic peptide C-AAALDLSGRRG from the C-terminus of human PPP1R15A / GADD34 (NP_055145.3). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon (91%); Trichomonas (82%).
LS-E25201 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C55303. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa664-674
Human PPP1R15A / GADD34.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About PPP1R15A / GADD34

O75807 NM_014330 NP_055145.3

PPP1R15A Antibody, GADD34 Antibody

Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.

Immunofluorescence

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong nuclear and some cytoplasmic staining. The
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong nuclear and some cytoplasmic staining. The

Immunofluorescence

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong nuclear and some cytoplasmic staining. The
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong nuclear and some cytoplasmic staining. The

Western blot

Antibody (0.1 ug/ml) staining of HEPG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.1 ug/ml) staining of HEPG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 5/28/2018

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