Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Rabbit Polyclonal to Human PPP1CB
Immunofluorescence, Western blot
Human, Mouse (tested or 100% immunogen sequence identity)
Mouse, Rat, Pig, Rabbit, Chicken, Xenopus, Cow (at least 90% immunogen sequence identity)
Ammonium sulfate precipitation
Immunofluorescence (1:10 - 1:50)
Western blot (1:1000)
Specificity and Use
LS-E9577 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101012. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
This Protein Phosphatase 1 beta (PPP1CB) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of human Protein Phosphatase 1 beta (PPP1CB).
PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity.
Fluorescent image of U251 cell stained with PPP1CB Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PPP1CB primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). PPP1CB immunoreactivity is localized to Cytoplasm and Nucleus significantly.
Western blot of PPP1CB (arrow) using rabbit polyclonal PPP1CB Antibody (K301). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPP1CB gene.