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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PPOX Antibody (aa35‑84) LS‑C111797
PPOX antibody LS-C111797 is an unconjugated rabbit polyclonal antibody to PPOX from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C111797-50
50 µg
Unavailable

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Product Description

PPOX antibody LS-C111797 is an unconjugated rabbit polyclonal antibody to PPOX from human, mouse, rat and other species. Validated for WB.
About PPOX
PPOX is the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified. P50336 NM_000309 NP_000300.1

PPOX Antibody, PPO Antibody, Protoporphyrinogen oxidase Antibody, V290M Antibody, Variegate porphyria Antibody, VP Antibody


Specifications

Target
Human PPOX
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Bat, Dog, Hamster (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
PPOX antibody was raised against synthetic peptide located between aa35-84 of human PPOX (P50336, NP_000300). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Bovine, Rabbit, Horse, Pig (100%); Hamster, Elephant, Dog, Bat, Opossum (92%); Salmon, Stickleback (91%); Guinea pig (85%).
Blocking Peptide
LS-E20309 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111797. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa35-84
Specificity
Human PPOX
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

PPOX antibody LS-C111797 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PPOX antibody LS-C111797 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PPOX antibody LS-C111797 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PPOX antibody LS-C111797 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PPOX antibody LS-C111797 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PPOX antibody LS-C111797 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PPOX antibody LS-C111797 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PPOX antibody LS-C111797 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/23/2018

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