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Anti-PPOX Antibody (aa35-84) LS-C111797

Catalog	Size	Price
LS-C111797-50	50 µg	Unavailable

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27 PPOX Antibodies

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Format:	Unconjugated

Immunoperoxidase of monoclonal antibody to PPOX on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 3 ug/ml].

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Immunohistochemistry of paraffin-embedded human lung cancer tissue.

Anti-PPOX Antibody LS-C406696

Antibody:	Rabbit Polyclonal (IgG) to Human PPOX
Reactivity:	Human, Mouse
Application:	IHC, Western blot, ELISA
Format:	Unconjugated

Immunohistochemistry of Human breast cancer using PPOX Polyclonal Antibody at dilution of 1:40.

Anti-PPOX Antibody LS-C412355

Antibody:	Rabbit Polyclonal to Human PPOX
Reactivity:	Human, Mouse, Rat
Application:	Immunofluorescence, Western blot
Format:	Unconjugated

Anti-PPOX Antibody LS-C482595

Antibody:	Rabbit Polyclonal to Human PPOX
Reactivity:	Human, Mouse, Rat
Application:	IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Format:	Unconjugated

Immunohistochemical analysis of PPOX staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Antibody:	Rabbit Polyclonal to Human PPOX
Reactivity:	Human, Monkey, Mouse, Rat, Bovine, Horse, Pig, Rabbit
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

PPOX antibody LS-C111797 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Details

Target:	Human PPOX
Host:	Rabbit
Reactivity:	Human, Monkey, Mouse, Rat, Bovine, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted:	Bat, Dog, Hamster (at least 90% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.2 - 1 µg/ml)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

PPOX antibody was raised against synthetic peptide located between aa35-84 of human PPOX (P50336, NP_000300). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Bovine, Rabbit, Horse, Pig (100%); Hamster, Elephant, Dog, Bat, Opossum (92%); Salmon, Stickleback (91%); Guinea pig (85%).

Blocking Peptide:

LS-E20309 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111797. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa35-84

Specificity:

Human PPOX

Usage:

Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About PPOX

Uniprot: P50336 NCBI: NM_000309 NP_000300.1

Synonyms: PPOX Antibody, PPO Antibody, Protoporphyrinogen oxidase Antibody, V290M Antibody, Variegate porphyria Antibody, VP Antibody

Description: PPOX is the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified.

(More About PPOX)

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Western blot

PPOX antibody LS-C111797 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 4/21/2018

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