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PPOX Antibody (aa35‑84) LS‑C111797
PPOX antibody LS-C111797 is an unconjugated rabbit polyclonal antibody to PPOX from human, mouse, rat and other species. Validated for WB.
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![Immunoperoxidase of monoclonal antibody to PPOX on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 3 ug/ml].](https://lsbio-7d62.kxcdn.com//image2/ppox-antibody-clone-2f10-ls-c133391/51481_1165151.jpg)
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, ICC, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
IHC, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Product Description
PPOX antibody LS-C111797 is an unconjugated rabbit polyclonal antibody to PPOX from human, mouse, rat and other species. Validated for WB.
About PPOX
PPOX is the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified.
P50336
NM_000309
NP_000300.1
PPOX Antibody, PPO Antibody, Protoporphyrinogen oxidase Antibody, V290M Antibody, Variegate porphyria Antibody, VP Antibody
Specifications
Target
Human PPOX
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Predicted
Bat, Dog, Hamster (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
Immunogen
PPOX antibody was raised against synthetic peptide located between aa35-84 of human PPOX (P50336, NP_000300). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Bovine, Rabbit, Horse, Pig (100%); Hamster, Elephant, Dog, Bat, Opossum (92%); Salmon, Stickleback (91%); Guinea pig (85%).
Blocking Peptide
LS-E20309 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111797. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-C111797. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa35-84
Specificity
Human PPOX
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Western blot
PPOX antibody LS-C111797 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
PPOX antibody LS-C111797 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
PPOX antibody LS-C111797 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
PPOX antibody LS-C111797 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From:
Date Requested: 6/23/2018
Date Requested: 6/23/2018
