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Anti-PPOX Antibody (aa35-84) LS-C111797

Catalog	Size	Price
LS-C111797-50	50 µg	Unavailable

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Antibody:	Mouse Monoclonal [clone 2F10] (IgG1,k) to Human PPOX
Reactivity:	Human
Application:	IHC - Paraffin, Western blot, ELISA
Format:	Unconjugated

Anti-PPOX Antibody (aa378-478, AP) LS-C281138

Antibody:	Mouse Monoclonal (IgG1) to Human PPOX
Reactivity:	Human
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Anti-PPOX Antibody LS-C334683

Antibody:	Rabbit Polyclonal (IgG) to Human PPOX
Reactivity:	Human, Mouse, Rat
Application:	IHC, Immunofluorescence, Western blot
Format:	Unconjugated

Anti-PPOX Antibody LS-C406696

Antibody:	Rabbit Polyclonal (IgG) to Human PPOX
Reactivity:	Human, Mouse
Application:	IHC, Western blot, ELISA
Format:	Unconjugated

Anti-PPOX Antibody LS-C482595

Antibody:	Rabbit Polyclonal to Human PPOX
Reactivity:	Human, Mouse, Rat
Application:	IHC - Paraffin, ICC, Immunofluorescence, Western blot
Format:	Unconjugated

Antibody:	Rabbit Polyclonal to Human PPOX
Reactivity:	Human, Monkey, Mouse, Rat, Bovine, Horse, Pig, Rabbit
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Details

Target:	Human PPOX
Host:	Rabbit
Reactivity:	Human, Monkey, Mouse, Rat, Bovine, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted:	Bat, Dog, Hamster (at least 90% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.2 - 1 µg/ml)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

PPOX antibody was raised against synthetic peptide located between aa35-84 of human PPOX (P50336, NP_000300). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Bovine, Rabbit, Horse, Pig (100%); Hamster, Elephant, Dog, Bat, Opossum (92%); Salmon, Stickleback (91%); Guinea pig (85%).

Blocking Peptide:

LS-E20309 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111797. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa35-84

Specificity:

Human PPOX

Usage:

Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About PPOX

Uniprot: P50336 NCBI: NM_000309 NP_000300.1

Synonyms: PPOX Antibody, PPO Antibody, Protoporphyrinogen oxidase Antibody, V290M Antibody, Variegate porphyria Antibody, VP Antibody

Description: PPOX is the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified.

(More About PPOX)

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Technical Help Review

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Western blot

PPOX antibody LS-C111797 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 6/22/2017

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