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Anti-PPM1B Antibody (aa288-479, clone K1B1) LS-C93786

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $315
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PPM1B Antibodies

Anti-PPM1B Antibody (C-Terminus) LS-C91551
Rabbit Polyclonal to Human PPM1B
Human
Western blot, Immunoprecipitation
Unconjugated
Anti-PPM1B Antibody (aa1-479) LS-C131253
Rabbit Polyclonal to Human PPM1B
Human
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone K1B1] (IgG2b,k) to Human PPM1B
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human PPM1B
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [K1B1]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (20 µg/ml)
  • Western blot (1:1000 - 1:3000)
  • ELISA

Specificity and Use

PPM1B antibody was raised against recombinant human PPM1B (288-479aa) purified from E. coli.
aa288-479
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since applications vary, each investigator should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000-1:3000.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About PPM1B

O75688 NM_002706 NP_002697.1

PPM1B Antibody, Phosphatase 2c beta Antibody, PPC2BETAX Antibody, PP2C-beta Antibody, Protein phosphatase 2A beta Antibody, Protein phosphatase 1B Antibody, PP2CB Antibody, PP2CBETA Antibody, PP2C-beta-X Antibody

PPM1B is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms.

Western blot

Western blot
Cell lysates of MCF7 (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1B (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
 

Requested From: United States
Date Requested: 12/7/2016

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