Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 1A3-2A4] (IgG1,k) to Human PPM1B
Immunofluorescence, Western blot, Immunoprecipitation, ELISA, RNA interference
Mouse Monoclonal [clone K1B1] (IgG2b,k) to Human PPM1B
IHC - Paraffin, Western blot, ELISA
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [K1B1]
Protein G purified
IHC - Paraffin (20 µg/ml)
Western blot (1:1000 - 1:3000)
Specificity and Use
PPM1B antibody was raised against recombinant human PPM1B (288-479aa) purified from E. coli.
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since applications vary, each investigator should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000-1:3000.
PBS, pH 7.4, 0.1% sodium azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
PPM1B is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms.
Cell lysates of MCF7 (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1B (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.