Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone K2E2] (IgG1) to Human PPIB / Cyclophilin B
Western blot, ELISA
Human PPIB / Cyclophilin B
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [K2E2]
Protein G purified
Western blot (1:1000 - 1:2000)
IHC - Paraffin
Specificity and Use
Recombinant human Cyclophilin B (26-216aa) purified from E. coli.
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000-1:2000.
PBS, pH 7.4, 0.1% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
PPIB Antibody, Cyclophilin B Antibody, CYP-S1 Antibody, S-cyclophilin Antibody, Rotamase B Antibody, Cyclophilin-like protein Antibody, CYPB Antibody, OI9 Antibody, PPIase B Antibody, SCYLP Antibody
PPIB / Cyclophilin B is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta.
Cell lysates of HepG2(30 ug) and HeLa(30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cyclophilin B (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection.