Human PPARG / PPAR Gamma
Human, Monkey, Mouse, Rat, Bat, Dog, Hamster, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot (1 - 5 µg/ml)
- (applications tested for the base form of this product only)
Synthetic peptide (GEDKIKFKHITPLQEQ) corresponding to aa256-271 of human PPARg1 and aa287-302 of human PPARg2 (KLH). The immunizing sequence is identical in mouse and rat. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Porcine, Cat, Bat, Dog, Horse, Rabbit, Opossum (100%); Elephant, Platypus (94%); Sheep, Goat, Bovine (88%).
Recognizes human and mouse PPARg1 and 2, Mr ~55-60kD. A non-specific protein was also detected, Mr ~47kD after longer exposure. Species sequence homology: Rat, porcine, rabbit, rhesus monkey and mink.
Suitable for use in Western Blot. Western Blot: 1-5 ug/ml of this lot detected PPARg protein in RIPA lysates from 3T3/L1 cells differentiated into adipocytes but not in undifferentiated 3T3/L1 cells.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: -20°C; Long term: -20°C.
For research use only.