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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PPARG / PPAR Gamma Antibody (aa170‑270, clone 3A4A9) IHC‑plus™ LS‑B3892
Note: This antibody replaces LS-C108880
PPAR Gamma antibody LS-B3892 is an unconjugated mouse monoclonal antibody to human PPAR Gamma (PPARG). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B3892-50
50 µl
Unavailable

Popular PPARG / PPAR Gamma Products

Anti-PPARG antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B130 concentration 5 ug/ml.
Species: Human, Macaque
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-PPARG antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B651 concentration 5 ug/ml.
Species: Human, Mouse, Rat, Dog, Guinea pig, Hamster, Mink, Rabbit, Boar, Duck, Macaque, Squirrel
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-PPARG antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7391 concentration 10 ug/ml.
Species: Human
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PPARG Antibody flow cytometry of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Species: Human
Applications: Western blot, Flow Cytometry
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

PPAR Gamma antibody LS-B3892 is an unconjugated mouse monoclonal antibody to human PPAR Gamma (PPARG). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About PPARG / PPAR Gamma
Peroxisome proliferator-activated receptor gamma (PPAR gamma), a NR1 Thyroid Hormone-Like Receptor, is a transcription factor that regulates genes involved in lipid metabolism and adipocyte differentiation and has been shown to affect placental differentiation and cell proliferation and differentiation pathways in various diseases such as Type II diabetes and atherosclerosis. Recently, PPAR gamma has been suggested to affect inflammatory digestive diseases (Dubuquoy et al. 2002). P37231 NM_015869 NP_056953.2

PPARG Antibody, NR1C3 Antibody, PPAR gamma Antibody, PPAR gamma 2 Antibody, PPARG1 Antibody, PPARG2 Antibody, PPARgamma Antibody, CIMT1 Antibody, GLM1 Antibody, PPAR-gamma Antibody


Specifications

Target
Human PPARG / PPAR Gamma
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [3A4A9]
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200 - 1:400)
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified recombinant fragment of PPARG (aa170-270) expressed in E. Coli.
Epitope
aa170-270
Specificity
Human
Usage
Immunohistochemistry: LS-B3892 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3892 was determined to be 1:200.
Presentation
Ascitic fluid containing 0.03% sodium azide
Storage
Short term: 4°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-PPARG antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3892 dilution 1:200.
Anti-PPARG antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3892 dilution 1:200.

Immunohistochemistry

Anti-PPARG antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3892 dilution 1:200.
Anti-PPARG antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3892 dilution 1:200.

Western blot

Western blot using PPARG mouse monoclonal antibody against truncated PPARG-His recombinant protein (1) and full-length PPARG(aa1-477) transfected CHO-K1 cell lysate (2).
Western blot using PPARG mouse monoclonal antibody against truncated PPARG-His recombinant protein (1) and full-length PPARG(aa1-477) transfected CHO-K1 cell lysate (2).

Immunohistochemistry

Anti-PPARG antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3892 dilution 1:200.
Anti-PPARG antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3892 dilution 1:200.

Immunohistochemistry

Anti-PPARG antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3892 dilution 1:200.
Anti-PPARG antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3892 dilution 1:200.

Western blot

Western blot using PPARG mouse monoclonal antibody against truncated PPARG-His recombinant protein (1) and full-length PPARG(aa1-477) transfected CHO-K1 cell lysate (2).
Western blot using PPARG mouse monoclonal antibody against truncated PPARG-His recombinant protein (1) and full-length PPARG(aa1-477) transfected CHO-K1 cell lysate (2).

Immunohistochemistry

Anti-PPARG antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3892 dilution 1:200.
Anti-PPARG antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3892 dilution 1:200.

Immunohistochemistry

Anti-PPARG antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3892 dilution 1:200.
Anti-PPARG antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3892 dilution 1:200.

Western blot

Western blot using PPARG mouse monoclonal antibody against truncated PPARG-His recombinant protein (1) and full-length PPARG(aa1-477) transfected CHO-K1 cell lysate (2).
Western blot using PPARG mouse monoclonal antibody against truncated PPARG-His recombinant protein (1) and full-length PPARG(aa1-477) transfected CHO-K1 cell lysate (2).

Immunohistochemistry

Anti-PPARG antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3892 dilution 1:200.
Anti-PPARG antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3892 dilution 1:200.

Immunohistochemistry

Anti-PPARG antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3892 dilution 1:200.
Anti-PPARG antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3892 dilution 1:200.

Western blot

Western blot using PPARG mouse monoclonal antibody against truncated PPARG-His recombinant protein (1) and full-length PPARG(aa1-477) transfected CHO-K1 cell lysate (2).
Western blot using PPARG mouse monoclonal antibody against truncated PPARG-His recombinant protein (1) and full-length PPARG(aa1-477) transfected CHO-K1 cell lysate (2).


Requested From: 
Date Requested: 6/18/2018

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