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Anti-PP2CB / PPP2CB Antibody (aa295-309) LS-C38583

Ordering

Wt. Vol. Conc. Price
- 200 µl - $515
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PP2CB / PPP2CB Antibodies

Anti-PP2CB / PPP2CB Antibody (aa278-307, APC) LS-C284723
Rabbit Polyclonal (IgG) to Human PP2CB / PPP2CB
Human
IHC, Western blot, ELISA
APC Conjugated
Anti-PP2CB / PPP2CB Antibody IHC-plus™ LS-B11253
Rabbit Polyclonal (IgG) to Human PP2CB / PPP2CB
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal to Human PP2CB / PPP2CB
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish, Drosophila, Insect
Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human PP2CB / PPP2CB
Mouse
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish, Drosophila, Insect (tested or 100% immunogen sequence identity)
Monoclonal
Unconjugated
Ascites
Unmodified

Applications

  • Western blot
  • Immunoprecipitation
  • ELISA

Specificity and Use

PP2CB / PPP2CB antibody was raised against kLH-conjugated, synthetic peptide C-RGEPHVTRRTPDYFL corresponding to residues 295-309 of human PP2A, C subunit, with cysteine. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus, Catfish, Salmon, Smelt, Stickleback, Pufferfish, Zebrafish, Seq squirt, Drosophila, Mosquito, Ant, Water flea, Slime mold, Nematode, Capsaspora (100%).
aa295-309
Recognizes unmethylated PP2A, C subunit, Mr 36kD. Species cross-reactivity: Predicted to cross-react with mouse, rat, bovine, porcine, rabbit and chicken due to sequence homology.
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Immunoprecipitation: Reported to immunoprecipitate unmethylated PP2A, C subunit.1. Western Blot Analysis: A 1:1000 dilution detects PP2A, demethylated in RIPA lysates from A431 cells. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PP2A, demethylated (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Packaging

200 ul of ascites diluted, 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About PP2CB / PPP2CB

P62714 NM_004156 NP_004147.1

PPP2CB Antibody, PP2Abeta Antibody, Protein phosphatase 2A beta Antibody, PP2CB Antibody, PP2A-beta Antibody, pp2a2 Antibody

PP2CB / PPP2CB is the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit.

Requested From: United States
Date Requested: 12/7/2016

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