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Anti-PP2CB / PPP2CB Antibody (aa295-309) LS-C38583
Most Popular PP2CB / PPP2CB Antibodies
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Details
| Human PP2CB / PPP2CB | |
| Mouse | |
| Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish, Drosophila, Insect (tested or 100% immunogen sequence identity) | |
| IgG Monoclonal | |
| Unconjugated | |
| Ascites | |
| Unmodified |
Applications
- Western blot
- Immunoprecipitation
- ELISA
Specificity and Use
| PP2CB / PPP2CB antibody was raised against kLH-conjugated, synthetic peptide C-RGEPHVTRRTPDYFL corresponding to residues 295-309 of human PP2A, C subunit, with cysteine. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus, Catfish, Salmon, Smelt, Stickleback, Pufferfish, Zebrafish, Seq squirt, Drosophila, Mosquito, Ant, Water flea, Slime mold, Nematode, Capsaspora (100%). | |
| aa295-309 | |
| Recognizes unmethylated PP2A, C subunit, Mr 36kD. Species cross-reactivity: Predicted to cross-react with mouse, rat, bovine, porcine, rabbit and chicken due to sequence homology. | |
| Suitable for use in ELISA, Immunoprecipitation and Western Blot. Immunoprecipitation: Reported to immunoprecipitate unmethylated PP2A, C subunit.1. Western Blot Analysis: A 1:1000 dilution detects PP2A, demethylated in RIPA lysates from A431 cells. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PP2A, demethylated (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. |
Packaging
| 200 ul of ascites diluted, 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol. | |
| Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted. | |
| For research use only. |
About PP2CB / PPP2CB
P62714
NM_004156
NP_004147.1
PPP2CB Antibody, PP2Abeta Antibody, Protein phosphatase 2A beta Antibody, PP2CB Antibody, PP2A-beta Antibody, pp2a2 Antibody
PP2CB / PPP2CB is the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit.
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Requested From:
Date Requested: 4/19/2018
Date Requested: 4/19/2018
