Human PP2CB / PPP2CB
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish, Drosophila, Insect
(tested or 100% immunogen sequence identity)
- Western blot
PP2CB / PPP2CB antibody was raised against kLH-conjugated, synthetic peptide C-RGEPHVTRRTPDYFL corresponding to residues 295-309 of human PP2A, C subunit, with cysteine. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus, Catfish, Salmon, Smelt, Stickleback, Pufferfish, Zebrafish, Seq squirt, Drosophila, Mosquito, Ant, Water flea, Slime mold, Nematode, Capsaspora (100%).
Recognizes unmethylated PP2A, C subunit, Mr 36kD. Species cross-reactivity: Predicted to cross-react with mouse, rat, bovine, porcine, rabbit and chicken due to sequence homology.
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Immunoprecipitation: Reported to immunoprecipitate unmethylated PP2A, C subunit.1. Western Blot Analysis: A 1:1000 dilution detects PP2A, demethylated in RIPA lysates from A431 cells. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PP2A, demethylated (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
200 ul of ascites diluted, 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
For research use only.
This antibody carries the LSBio 100% Guarantee.