LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
POU6F1 / BRN5 Antibody (aa72‑83) LS‑C4235
BRN5 antibody LS-C4235 is an unconjugated rabbit polyclonal antibody to BRN5 (POU6F1) from human, rat, bat and other species. Validated for ELISA and WB.
Catalog
Size
Price
LS-C4235-100
100 µg
$375
Description
BRN5 antibody LS-C4235 is an unconjugated rabbit polyclonal antibody to BRN5 (POU6F1) from human, rat, bat and other species. Validated for ELISA and WB.
Target
Human POU6F1 / BRN5
Host
Rabbit
Reactivity
Human, Monkey, Rat, Bat, Bovine, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Mouse, Dog (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1:200 - 1:1000)
  • ELISA
Immunogen
POU6F1 / BRN5 antibody was raised against synthetic peptide from human Brn-5, aa72-83, KPSTPESPAKSE (KLH). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Rat, Elephant, Bovine, Bat, Horse, Rabbit, Pig (100%); Mouse, Panda, Dog (92%); Lizard, Seq squirt, Rice, Trichomonas (83%).
Epitope
aa72-83
Specificity
Recognizes human Brn-5/Pou6F1, a POU domain transcription factor. Species cross-reactivity: Rodent.
Usage
Suitable for use in ELISA and Western Blot. ELISA (serum titer): 1:50000. Western Blot: HepG2:38, 42, 54kD. Human hippocampus: 38, 42, 54, 100kD. Human thalamus: 38, 42kD. Rat brain: 42, 54kD. Mouse brain: 42, 54kD. Human brain: 42kD. Human liver: 38, 54kD.
Presentation
PBS, 0.01% sodium azide, 40% glycerol.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About POU6F1 / BRN5
P56223 L23204 P56223

Popular POU6F1 / BRN5 Products

Fetal Kidney Nu. Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Rabbit, Xenopus
Applications: Western blot
Immunofluorescent analysis of Brn-5 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot
Antibody
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Immunofluorescence analysis of MCF7 cells.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Western Blot analysis of POU6F1 expression in transfected 293T cell line by POU6F1 monoclonal antibody (M01), clone 6H1.Lane 1: POU6F1 transfected lysate(32.6 KDa).Lane 2: Non-transfected lysate.
Species: Human
Applications: Western blot, ELISA, RNA interference

Publications (0)


Customer Reviews (0)


Requested From: United States
Date Requested: 12/15/2018
Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy