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Anti-POU6F1 / BRN5 Antibody (aa72-83) LS-C4235

Catalog Size Price
LS-C4235-100 100 µg Unavailable

Related Products

25 POU6F1 / BRN5 Antibodies

Most Popular POU6F1 / BRN5 Antibodies

Anti-POU6F1 / BRN5 Antibody (C-Terminus) LS-C29935
Rabbit Polyclonal (IgG) to Human POU6F1 / BRN5
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Rabbit, Xenopus
Western blot
Unconjugated
Fetal Kidney Nu. Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-POU6F1 / BRN5 Antibody (clone 6H1) LS-C197956
Mouse Monoclonal [clone 6H1] (IgG2a,k) to Human POU6F1 / BRN5
Human
Western blot, ELISA, RNA interference
Unconjugated
Western Blot analysis of POU6F1 expression in transfected 293T cell line by POU6F1 monoclonal antibody (M01), clone 6H1.Lane 1: POU6F1 transfected lysate(32.6 KDa).Lane 2: Non-transfected lysate.
Anti-POU6F1 / BRN5 Antibody LS-C346344
Rabbit Polyclonal (IgG) to Human POU6F1 / BRN5
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence analysis of MCF7 cells.
Anti-POU6F1 / BRN5 Antibody LS-C412838
Rabbit Polyclonal to Human POU6F1 / BRN5
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated
Anti-POU6F1 / BRN5 Antibody LS-C482796
Rabbit Polyclonal to Human POU6F1 / BRN5
Human, Mouse, Rat
ICC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescent analysis of Brn-5 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

100% Guaranteed
Rabbit Polyclonal (IgG) to Human POU6F1 / BRN5
Human, Monkey, Rat, Bat, Bovine, Horse, Pig, Rabbit
Western blot, ELISA
Unconjugated

Details

Human POU6F1 / BRN5
Rabbit
Human, Monkey, Rat, Bat, Bovine, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Mouse, Dog (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • Western blot (1:200 - 1:1000)
  • ELISA

Specificity and Use

POU6F1 / BRN5 antibody was raised against synthetic peptide from human Brn-5, aa72-83, KPSTPESPAKSE (KLH). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Rat, Elephant, Bovine, Bat, Horse, Rabbit, Pig (100%); Mouse, Panda, Dog (92%); Lizard, Seq squirt, Rice, Trichomonas (83%).
aa72-83
Recognizes human Brn-5/Pou6F1, a POU domain transcription factor. Species cross-reactivity: Rodent.
Suitable for use in ELISA and Western Blot. ELISA (serum titer): 1:50000. Western Blot: HepG2:38, 42, 54kD. Human hippocampus: 38, 42, 54, 100kD. Human thalamus: 38, 42kD. Rat brain: 42, 54kD. Mouse brain: 42, 54kD. Human brain: 42kD. Human liver: 38, 54kD.

Packaging

PBS, 0.01% sodium azide, 40% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About POU6F1 / BRN5

P56223 L23204 P56223

POU6F1 Antibody, Brn-5 Antibody, TCFB1 Antibody, MPOU homeobox protein Antibody, Brain-5 Antibody, BRN5 Antibody, MPOU Antibody, POU class 6 homeobox 1 Antibody

Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3').

Requested From: 
Date Requested: 5/21/2018

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